Mosckovitz_1987_Biochem.Biophys.Res.Commun_145_810

Reference

Title : Localization of azidophencyclidine-binding site on the nicotinic acetylcholine receptor alpha-subunit - Mosckovitz_1987_Biochem.Biophys.Res.Commun_145_810
Author(s) : Mosckovitz R , Haring R , Gershoni JM , Kloog Y , Sokolovsky M
Ref : Biochemical & Biophysical Research Communications , 145 :810 , 1987
Abstract :

Nicotinic acetylcholine receptors in receptor-rich membranes from Torpedo californica and from T. marmorata electric tissue were photolabeled with the non-competitive inhibitor [3H]azidophencyclidine. The receptor subunits were separated on SDS-polyacrylamide gels and the alpha-subunits recovered from the gel, were subjected to Staphylococcus aureus V8 protease cleavage. The proteolytic fragments were resolved by SDS-polyacrylamide gel electrophoresis and were identified on protein blots by 125I-labeled alpha-bungarotoxin binding and by staining with concanavalin A. The site of specific azidophencyclidine labeling has been localized to the V8-18 kDa fragment which binds toxin. Labeling of the V8-18 kDa fragment was observed in the absence and in the presence of carbamylcholine. This was found for both the species of Torpedo used here.

PubMedSearch : Mosckovitz_1987_Biochem.Biophys.Res.Commun_145_810
PubMedID: 3297059

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Citations formats

Mosckovitz R, Haring R, Gershoni JM, Kloog Y, Sokolovsky M (1987)
Localization of azidophencyclidine-binding site on the nicotinic acetylcholine receptor alpha-subunit
Biochemical & Biophysical Research Communications 145 :810

Mosckovitz R, Haring R, Gershoni JM, Kloog Y, Sokolovsky M (1987)
Biochemical & Biophysical Research Communications 145 :810