Title : Localization of azidophencyclidine-binding site on the nicotinic acetylcholine receptor alpha-subunit - Mosckovitz_1987_Biochem.Biophys.Res.Commun_145_810 |
Author(s) : Mosckovitz R , Haring R , Gershoni JM , Kloog Y , Sokolovsky M |
Ref : Biochemical & Biophysical Research Communications , 145 :810 , 1987 |
Abstract :
Nicotinic acetylcholine receptors in receptor-rich membranes from Torpedo californica and from T. marmorata electric tissue were photolabeled with the non-competitive inhibitor [3H]azidophencyclidine. The receptor subunits were separated on SDS-polyacrylamide gels and the alpha-subunits recovered from the gel, were subjected to Staphylococcus aureus V8 protease cleavage. The proteolytic fragments were resolved by SDS-polyacrylamide gel electrophoresis and were identified on protein blots by 125I-labeled alpha-bungarotoxin binding and by staining with concanavalin A. The site of specific azidophencyclidine labeling has been localized to the V8-18 kDa fragment which binds toxin. Labeling of the V8-18 kDa fragment was observed in the absence and in the presence of carbamylcholine. This was found for both the species of Torpedo used here. |
PubMedSearch : Mosckovitz_1987_Biochem.Biophys.Res.Commun_145_810 |
PubMedID: 3297059 |
Mosckovitz R, Haring R, Gershoni JM, Kloog Y, Sokolovsky M (1987)
Localization of azidophencyclidine-binding site on the nicotinic acetylcholine receptor alpha-subunit
Biochemical & Biophysical Research Communications
145 :810
Mosckovitz R, Haring R, Gershoni JM, Kloog Y, Sokolovsky M (1987)
Biochemical & Biophysical Research Communications
145 :810