Moss_1991_J.Mol.Neurosci_3_101

Reference

Title : Evidence that CGRP and cAMP increase transcription of AChR alpha-subunit gene, but not of other subunit genes - Moss_1991_J.Mol.Neurosci_3_101
Author(s) : Moss SJ , Harkness PC , Mason IJ , Barnard EA , Mudge AW
Ref : Journal of Molecular Neuroscience , 3 :101 , 1991
Abstract :

Calcitonin gene-related peptide (CGRP) is present in embryonic chick motoneurons and their terminals during myogenesis. We have studied the effect of CGRP on the expression of mRNA encoding the four subunits (alpha, beta, gamma, delta) of ACh receptors in cultured myotubes derived from embryonic chicks. Northern blot analysis showed that treatment with 10(-7) M CGRP caused an increase in ACh receptor alpha-subunit mRNA expression but did not affect the expression of beta-, gamma-, or delta-subunit mRNAs. In addition, CGRP treatment caused an increase in the expression of unspliced alpha-subunit RNA, suggesting that CGRP increases transcription of the alpha-subunit gene. The effect of CGRP on alpha-subunit gene transcription was mimicked by forskolin, and both CGRP and forskolin increased the levels of intracellular cAMP. We infer that the effect of CGRP on alpha-subunit gene transcription is likely to be mediated by the CGRP-induced rise in intracellular cAMP.

PubMedSearch : Moss_1991_J.Mol.Neurosci_3_101
PubMedID: 1687655

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Citations formats

Moss SJ, Harkness PC, Mason IJ, Barnard EA, Mudge AW (1991)
Evidence that CGRP and cAMP increase transcription of AChR alpha-subunit gene, but not of other subunit genes
Journal of Molecular Neuroscience 3 :101

Moss SJ, Harkness PC, Mason IJ, Barnard EA, Mudge AW (1991)
Journal of Molecular Neuroscience 3 :101