| Title : Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells: evidence for isozyme variants - Mullen_1999_FEBS.Lett_445_301 |
| Author(s) : Mullen RT , Trelease RN , Duerk H , Arand M , Hammock BD , Oesch F , Grant DF |
| Ref : FEBS Letters , 445 :301 , 1999 |
| Abstract : Endogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal lie of mouse soluble epoxide hydrolase was mutated to generate a prototypic putative type 1 PTS (-SKI to -SKL), the enzyme targeted to peroxisomes. The possibility that soluble epoxide hydrolase-SKI was sorted slowly to peroxiosmes from the cytosol was examined by stably expressing rat soluble epoxide hydrolase-SKI appended to the green fluorescent protein. Green fluorescent protein soluble epoxide hydrolase-SKI was strictly cytosolic, indicating that -SKI was not a temporally inefficient putative type 1 PTS. Import of soluble epoxide hydrolase-SKI into peroxisomes in plant cells revealed that the context of -SKI on soluble epoxide hydrolase was targeting permissible. These results show that the C-terminal -SKI is a non-functional putative type 1 PTS on soluble epoxide hydrolase and suggest the existence of distinct cytosolic and peroxisomal targeting variants of soluble epoxide hydrolase in mouse and rat. |
| ESTHER : Mullen_1999_FEBS.Lett_445_301 |
| PubMedSearch : Mullen_1999_FEBS.Lett_445_301 |
| PubMedID: 10094477 |
Mullen RT, Trelease RN, Duerk H, Arand M, Hammock BD, Oesch F, Grant DF (1999)
Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells: evidence for isozyme variants
FEBS Letters
445 :301
Mullen RT, Trelease RN, Duerk H, Arand M, Hammock BD, Oesch F, Grant DF (1999)
FEBS Letters
445 :301