Nagao_1997_J.Chromatogr.B.Biomed.Sci.Appl_701_9

Reference

Title : Detection of sarin hydrolysis products from sarin-like organophosphorus agent-exposed human erythrocytes - Nagao_1997_J.Chromatogr.B.Biomed.Sci.Appl_701_9
Author(s) : Nagao M , Takatori T , Matsuda Y , Nakajima M , Niijima H , Iwase H , Iwadate K , Amano T
Ref : Journal of Chromatography B Biomed Sci Appl , 701 :9 , 1997
Abstract : A sarin-like organophosphorus agent, [bis(isopropyl methyl)phosphonate; BIMP], was synthesized. This agent has the same phosphonate group as sarin and also has the same anti-acetylcholinesterase activity potency as sarin. The ID50 and LD50 values of BIMP in mice after intravenous injection were 3.9 nM and 0.8 mg/kg, respectively. The AChE activities of their red blood cells and brains were dose-dependently reduced by intravenous BIMP. After preparation of experimental BIMP-exposed human red blood cells, BIMP-bound acetylcholinesterase (AChE) was solubilized from erythrocyte membranes, purified by immunoaffinity chromatography, digested with trypsin, and the sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion. The digested sarin hydrolysis products were subjected to trimethylsilyl (TMS) derivatization and detected by gas chromatography-mass spectrometry. Isopropyl methylphosphonic- and methylphosphonic acids, which are the sarin hydrolysis products, were detected in experimental BIMP-exposed human red blood cells. This new method, which enables sarin's hydrolysis products to be detected in BIMP-exposed erythrocytes, is a useful tool for studying sarin-poisoning victims.
ESTHER : Nagao_1997_J.Chromatogr.B.Biomed.Sci.Appl_701_9
PubMedSearch : Nagao_1997_J.Chromatogr.B.Biomed.Sci.Appl_701_9
PubMedID: 9389333

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Citations formats

Nagao M, Takatori T, Matsuda Y, Nakajima M, Niijima H, Iwase H, Iwadate K, Amano T (1997)
Detection of sarin hydrolysis products from sarin-like organophosphorus agent-exposed human erythrocytes
Journal of Chromatography B Biomed Sci Appl 701 :9

Nagao M, Takatori T, Matsuda Y, Nakajima M, Niijima H, Iwase H, Iwadate K, Amano T (1997)
Journal of Chromatography B Biomed Sci Appl 701 :9