Nagao_1997_Toxicol.Appl.Pharmacol_144_198

Reference

Title : Definitive evidence for the acute sarin poisoning diagnosis in the Tokyo subway - Nagao_1997_Toxicol.Appl.Pharmacol_144_198
Author(s) : Nagao M , Takatori T , Matsuda Y , Nakajima M , Iwase H , Iwadate K
Ref : Toxicol Appl Pharmacol , 144 :198 , 1997
Abstract : A new method was developed to detect sarin hydrolysis products from erythrocytes of four victims of sarin (isopropylmethylphosphonofluoridate) poisoning resulting from the terrorist attack on the Tokyo subway. Sarin-bound acetylcholinesterase (AChE) was solubilized from erythrocyte membranes of sarin victims, digested with trypsin, the sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion, and the digested sarin hydrolysis products were subjected to trimethylsilyl derivatization and detected by gas chromatography-mass spectrometry. Isopropylmethylphosphonic acid, which is a sarin hydrolysis product, was detected in all sarin poisoning, victims we examined and methylphosphonic acid, which is a sarin and soman hydrolysis product, was determined in all victims. Postmortem examinations revealed no macroscopic and microscopic findings specific to sarin poisoning and sarin and its hydrolysis products were almost undetectable in their blood. We think that the procedure described below will be useful for the forensic diagnosis of acute sarin poisoning.
ESTHER : Nagao_1997_Toxicol.Appl.Pharmacol_144_198
PubMedSearch : Nagao_1997_Toxicol.Appl.Pharmacol_144_198
PubMedID: 9169085

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Citations formats

Nagao M, Takatori T, Matsuda Y, Nakajima M, Iwase H, Iwadate K (1997)
Definitive evidence for the acute sarin poisoning diagnosis in the Tokyo subway
Toxicol Appl Pharmacol 144 :198

Nagao M, Takatori T, Matsuda Y, Nakajima M, Iwase H, Iwadate K (1997)
Toxicol Appl Pharmacol 144 :198