Noor_2020_Polym.Degrad.Stab_174_109096

Pseudomonas aeruginosa strain S3 exhibited high esterolytic activity against poly (lactic acid) (PLA) at environmental temperature (~30C). A comprehensive study was conducted for optimization of physico-chemical parameters for high-throughput esterase production using PlackettBurman Design and Central Composite Design. PLA degradation by Pseudomonas aeruginosa strain S3 was confirmed during preliminary studies using Fourier Transform Infrared Spectroscopy (FTIR) and scanning electron microscopy (SEM). The date obtained from statistical optimization demonstrated interactive action of inoculum size with peptone, yeast extract and Tween 20 was more influential towards esterase activity. The following medium constituents were optimized for maximum esterase production [(g/L) Glucose, 0.8; Peptone, 10.5; Yeast extract, 15; MgSO4.7H2O, 0.3; Sodium citrate, 4; CaCl2, 1; NaCl, 1; FeSO4.7H2O, 1; Inoculum size 75mL and Tween 20, 3.8mL (v/v)]. The molecular weight of purified esterase was approximately 35kDa with apparent Km and Vmax values, i.e., Km 12.38M and Vmax 769.23 U/mg, respectively. The enzyme showed stability over a broad range of pH (310) and temperature (2040C). The activity of the purified enzyme was significantly enhanced by non-polar detergents (Tween 80 and Triton-X100), glycerol as well as both mono and divalent cations (Co+2 and K+1). Hydrolysis of PLA films with purified esterase released oligomers of medium chain length (n=613), reaffirming the biodegradation potential of Pseudomonas aeruginosa strain S3 against PLA. The results obtained from the study demonstrate the potential of our strain and its enzymes in development of high efficient system of PLA biodegradation and recovery processes.