Title : Molecular cloning and characterization of esterase-6, a serine hydrolase of Drosophila - Oakeshott_1987_Proc.Natl.Acad.Sci.U.S.A_84_3359 |
Author(s) : Oakeshott JG , Collet C , Phillis RW , Nielsen KM , Russell RJ , Chambers GK , Ross V , Richmond RC |
Ref : Proc Natl Acad Sci U S A , 84 :3359 , 1987 |
Abstract :
The Est-6 gene of Drosophila melanogaster was cloned by screening libraries with synthetic oligonucleotides corresponding to tryptic peptides from purified esterase-6 (Est-6) protein. cDNA clones were isolated that hybridized in situ to the site of Est-6 on chromosome 3 at 69A1. Inserts in putative Est-6 cDNA clones were 1.85 kilobases (kb) long, and blot hybridization analysis of electrophoretically fractionated RNA, using a cDNA clone as a probe, revealed two transcripts, of 1.68 and 1.83 kb. The two transcripts showed the same developmental profile as the Est-6 protein. Neither transcript was detected in an Est-6-null line. The cDNA fragment was homologous to a 2.3-kb EcoRI-BamHI fragment in genomic clones, and this region was interrupted by the 8-kb B104 transposable element in the Est-6-null line. Conceptual translation of the cDNA sequence revealed a protein of 548 residues with 19% sequence similarity to acetylcholinesterase from the Torpedo ray. |
PubMedSearch : Oakeshott_1987_Proc.Natl.Acad.Sci.U.S.A_84_3359 |
PubMedID: 3106966 |
Gene_locus related to this paper: drome-este6 |
Gene_locus | drome-este6 |
Oakeshott JG, Collet C, Phillis RW, Nielsen KM, Russell RJ, Chambers GK, Ross V, Richmond RC (1987)
Molecular cloning and characterization of esterase-6, a serine hydrolase of Drosophila
Proc Natl Acad Sci U S A
84 :3359
Oakeshott JG, Collet C, Phillis RW, Nielsen KM, Russell RJ, Chambers GK, Ross V, Richmond RC (1987)
Proc Natl Acad Sci U S A
84 :3359