Title : The lipid and protein content of cholinergic synaptic vesicles from the electric organ of Torpedo marmorata purified to constant composition: implications for vesicle structure - Ohsawa_1979_Brain.Res_161_447 |
Author(s) : Ohsawa K , Dowe GH , Morris SJ , Whittaker VP |
Ref : Brain Research , 161 :447 , 1979 |
Abstract :
The lipid, protein, acetylcholine and ATP content of cholinergic synaptic vesicles isolated from the richly innervated electric organ of Torpedo marmorata and purified to constant composition has been determined. The number of vesicles present in the preparations has been estimated by quantitative electron microscopy and the mean composition of the vesicle deduced. The acetylcholine content of the purest preparations was considerably greater than that previously attained and reached a mean of 6.10 mmole/g of protein and 2.6 X 10(5) molecules/vesicle; the mean values, for all determinations, were 4.1 +/- S.E.M. 0.6 and 2.6 X 10(5) +/- S.E.M. 0.6 X 10(5) respectively. |
PubMedSearch : Ohsawa_1979_Brain.Res_161_447 |
PubMedID: 421130 |
Ohsawa K, Dowe GH, Morris SJ, Whittaker VP (1979)
The lipid and protein content of cholinergic synaptic vesicles from the electric organ of Torpedo marmorata purified to constant composition: implications for vesicle structure
Brain Research
161 :447
Ohsawa K, Dowe GH, Morris SJ, Whittaker VP (1979)
Brain Research
161 :447