Okello_2012_Food.Funct_3_651

By 2034 it is forecast that 5 of the global population will be aged 85 years or over--approximately two and half fold increase on present day figures--which will inevitably lead to an increase in age-associated disorders such as Alzheimer's disease There is mounting evidence that green tea Camellia sinensis possesses numerous health-promoting properties and may potentially be beneficial to those suffering from Alzheimer's and other diseases including cardiovascular disease and cancer These beneficial properties are largely attributed to the high polyphenol content particularly the catechins In this study we measured acetylcholinesterase inhibition by white and green teas and their simulated intestinal digests We found that the potency with which the white and green tea extracts inhibited acetylcholinesterase varied through the simulated digestion procedure Initially in the undigested extract form potency was high with IC(5)(0 values of 7.20 mug mL(-)(1 and 8.06 mug mL(-)(1 for green and white tea respectively.However this decreased significantly after gastric digestion but activity was recovered after pancreatic digestion which could be related to relative increases in the levels of caffeine and specific phenolic components Of the pure tea compounds tested EGCG was the most potent with an IC(5)(0 of 0.0096 mumol mL(-)(1 but its breakdown product gamma-valerolactone was the least potent analyte Particularly interesting were the results of caffeine,which exhibited a strong inhibitory activity and pyrogallol which recorded a much stronger potency than its parent compound gallic acid suggesting a pro-drug-like relationship Overall the results indicate that further research is necessary to determine the full potential of digestion of tea and its metabolites and how inter-individual variation may indicate that some sections of society could potentially benefit more from drinking tea as a strategy to prevent the development of dementia We have also shown the activities of a number of metabolites,however further research is required to determine their potential bioavailability.