| Title : Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions - Olano_2004_Chem.Biol_11_87 |
| Author(s) : Olano C , Wilkinson B , Sanchez C , Moss SJ , Sheridan R , Math V , Weston AJ , Brana AF , Martin CJ , Oliynyk M , Mendez C , Leadlay PF , Salas JA |
| Ref : Chemical Biology , 11 :87 , 2004 |
|
Abstract :
The biosynthetic gene cluster for the angiogenesis inhibitor borrelidin has been cloned from Streptomyces parvulus Tu4055. Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption of borA3. The borrelidin PKS is striking because only seven rather than the nine modules expected for a nonaketide product are encoded by borA1-A6. The starter unit of the PKS has been verified as trans-cyclopentane-1,2-dicarboxylic acid (trans-1,2-CPDA), and the genes involved in its biosynthesis identified. Other genes responsible for biosynthesis of the nitrile moiety, regulation, and self-resistance were also identified. |
| PubMedSearch : Olano_2004_Chem.Biol_11_87 |
| PubMedID: 15112998 |
| Gene_locus related to this paper: 9acto-q70hz7 , 9acto-q70i09 , 9acto-q70i12 |
| Gene_locus | 9acto-q70hz7 9acto-q70i09 9acto-q70i12 |
| Gene_locus_frgt | 9acto-q70i05 |
Olano C, Wilkinson B, Sanchez C, Moss SJ, Sheridan R, Math V, Weston AJ, Brana AF, Martin CJ, Oliynyk M, Mendez C, Leadlay PF, Salas JA (2004)
Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions
Chemical Biology
11 :87
Olano C, Wilkinson B, Sanchez C, Moss SJ, Sheridan R, Math V, Weston AJ, Brana AF, Martin CJ, Oliynyk M, Mendez C, Leadlay PF, Salas JA (2004)
Chemical Biology
11 :87