| Title : Visual reading method for detection of bacterial tannase - Osawa_1993_Appl.Environ.Microbiol_59_1251 |
| Author(s) : Osawa R , Walsh TP |
| Ref : Applied Environmental Microbiology , 59 :1251 , 1993 |
|
Abstract :
Tannase activity of bacteria capable of degrading tannin-protein complexes was determined by a newly developed visual reading method. The method is based on two phenomena: (i) the ability of tannase to hydrolyze methyl gallate to release free gallic acid and (ii) the green to brown coloration of gallic acid after prolonged exposure to oxygen in an alkaline condition. The method has been successfully used to detect the presence of tannase in the cultures of bacteria capable of degrading tannin-protein complexes. |
| PubMedSearch : Osawa_1993_Appl.Environ.Microbiol_59_1251 |
| PubMedID: 16348918 |
| Substrate | Methyl-Gallate |
Osawa R, Walsh TP (1993)
Visual reading method for detection of bacterial tannase
Applied Environmental Microbiology
59 :1251
Osawa R, Walsh TP (1993)
Applied Environmental Microbiology
59 :1251