| Title : GTP-binding proteins in bovine brain nuclear membranes - Otto_1992_Neurochem.Int_21_409 |
| Author(s) : Otto H , Buchner K , Beckmann R , Hilbert R , Hucho F |
| Ref : Neurochem Int , 21 :409 , 1992 |
|
Abstract :
Nuclear membranes and other subcellular fractions derived from bovine brain cortex were investigated for the existence of GTP-binding proteins. By using photolytic labeling with [alpha-32P]GTP a 29 kDa GTP-binding protein was shown to be present in nuclear membranes which was not present in the plasma membranes nor in microsomal or cytosolic fractions. Two-dimensional gel electrophoresis revealed that this protein is rather acidic with a pI lower than 4.5. Members of the heterotrimeric Gi/o family are not present in the nuclear envelope: a 39 kDa protein, ADP ribosylated by pertussis toxin, was shown to originate from plasma membrane contamination. |
| PubMedSearch : Otto_1992_Neurochem.Int_21_409 |
| PubMedID: 1303165 |
Otto H, Buchner K, Beckmann R, Hilbert R, Hucho F (1992)
GTP-binding proteins in bovine brain nuclear membranes
Neurochem Int
21 :409
Otto H, Buchner K, Beckmann R, Hilbert R, Hucho F (1992)
Neurochem Int
21 :409