Title : Zebrafish developmental screening of the ToxCast Phase I chemical library - Padilla_2012_Reprod.Toxicol_33_174 |
Author(s) : Padilla S , Corum D , Padnos B , Hunter DL , Beam A , Houck KA , Sipes N , Kleinstreuer N , Knudsen T , Dix DJ , Reif DM |
Ref : Reprod Toxicol , 33 :174 , 2012 |
Abstract :
Zebrafish (Danio rerio) is an emerging toxicity screening model for both human health and ecology. As part of the Computational Toxicology Research Program of the U.S. EPA, the toxicity of the 309 ToxCast Phase I chemicals was assessed using a zebrafish screen for developmental toxicity. All exposures were by immersion from 6-8 h post fertilization (hpf) to 5 days post fertilization (dpf); nominal concentration range of 1 nM-80 muM. On 6 dpf larvae were assessed for death and overt structural defects. Results revealed that the majority (62%) of chemicals were toxic to the developing zebrafish; both toxicity incidence and potency was correlated with chemical class and hydrophobicity (logP); and inter-and intra-plate replicates showed good agreement. The zebrafish embryo screen, by providing an integrated model of the developing vertebrate, compliments the ToxCast assay portfolio and has the potential to provide information relative to overt and organismal toxicity. |
PubMedSearch : Padilla_2012_Reprod.Toxicol_33_174 |
PubMedID: 22182468 |
Padilla S, Corum D, Padnos B, Hunter DL, Beam A, Houck KA, Sipes N, Kleinstreuer N, Knudsen T, Dix DJ, Reif DM (2012)
Zebrafish developmental screening of the ToxCast Phase I chemical library
Reprod Toxicol
33 :174
Padilla S, Corum D, Padnos B, Hunter DL, Beam A, Houck KA, Sipes N, Kleinstreuer N, Knudsen T, Dix DJ, Reif DM (2012)
Reprod Toxicol
33 :174