| Title : Nanoparticle-Delivered 2-PAM for Rat Brain Protection against Paraoxon Central Toxicity - Pashirova_2017_ACS.Appl.Mater.Interfaces_9_16922 |
| Author(s) : Pashirova TN , Zueva IV , Petrov KA , Babaev VM , Lukashenko SS , Rizvanov IK , Souto EB , Nikolsky EE , Zakharova LY , Masson P , Sinyashin OG |
| Ref : ACS Appl Mater Interfaces , 9 :16922 , 2017 |
| Abstract : Solid lipid nanoparticles (SLNs) are among the most promising nanocarriers to target the blood-brain barrier (BBB) for drug delivery to the central nervous system (CNS). Encapsulation of the acetylcholinesterase reactivator, pralidoxime chloride (2-PAM), in SLNs appears to be a suitable strategy for protection against poisoning by organophosphorus agents (OPs) and postexposure treatment. 2-PAM-loaded SLNs were developed for brain targeting and delivery via intravenous (iv) administration. 2-PAM-SLNs displayed a high 2-PAM encapsulation efficiency ( approximately 90%) and loading capacity (maximum 30.8 +/- 1%). Drug-loaded particles had a mean hydrodynamic diameter close to 100 nm and high negative zeta potential (-54 to -15 mV). These properties contribute to improve long-term stability of 2-PAM-SLNs when stored both at room temperature (22 degrees C) and at 4 degrees C, as well as to longer circulation time in the bloodstream compared to free 2-PAM. Paraoxon-poisoned rats (2 x LD50) were treated with 2-PAM-loaded SLNs at a dose of 2-PAM of 5 mg/kg. 2-PAM-SLNs reactivated 15% of brain AChE activity. Our results confirm the potential use of SLNs loaded with positively charged oximes as a medical countermeasure both for protection against OPs poisoning and for postexposure treatment. |
| ESTHER : Pashirova_2017_ACS.Appl.Mater.Interfaces_9_16922 |
| PubMedSearch : Pashirova_2017_ACS.Appl.Mater.Interfaces_9_16922 |
| PubMedID: 28504886 |
Pashirova TN, Zueva IV, Petrov KA, Babaev VM, Lukashenko SS, Rizvanov IK, Souto EB, Nikolsky EE, Zakharova LY, Masson P, Sinyashin OG (2017)
Nanoparticle-Delivered 2-PAM for Rat Brain Protection against Paraoxon Central Toxicity
ACS Appl Mater Interfaces
9 :16922
Pashirova TN, Zueva IV, Petrov KA, Babaev VM, Lukashenko SS, Rizvanov IK, Souto EB, Nikolsky EE, Zakharova LY, Masson P, Sinyashin OG (2017)
ACS Appl Mater Interfaces
9 :16922