Perrier_2002_Neuron_33_275

As a tetramer acetylcholinesterase AChE is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses We have previously shown that collagen Q ColQ anchors AChE at the neuromuscular junction We have now cloned the gene PRiMA proline-rich membrane anchor encoding the AChE anchor in mammalian brain We show that PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells Furthermore we demonstrate that AChE is actually anchored in neural cell membranes through its interaction with PRiMA Finally we propose that only PRiMA anchors AChE in mammalian brain and muscle cell membranes