| Title : Identification of the dipeptidyl aminopeptidase responsible for N-terminal clipping of recombinant Exendin-4 precursor expressed in Pichia pastoris - Prabha_2009_Protein.Expr.Purif_64_155 |
| Author(s) : Prabha L , Govindappa N , Adhikary L , Melarkode R , Sastry K |
| Ref : Protein Expr Purif , 64 :155 , 2009 |
|
Abstract :
Exendin-4 is a naturally occurring 39 amino acid peptide that is useful for the control of Type 2 diabetes. Recombinant Exendin-4, with an extra glycine at the carboxy-terminus (Exdgly), was expressed in the methylotropic yeast Pichia pastoris. A high proportion of the Exdgly molecules secreted into medium were found to be clipped, lacking the first two amino acids (His-Gly) from the N-terminus. Disruption of the P. pastoris homolog of the Saccharomyces cerevisiae dipeptidyl aminopeptidase (STE13) gene in Pichia genome resulted in a clone that expressed N-terminally intact Exdgly. Elimination of N-terminal clipping enhanced the yield and simplified the purification of Exdgly from P. pastoris culture supernatant. |
| PubMedSearch : Prabha_2009_Protein.Expr.Purif_64_155 |
| PubMedID: 19028585 |
| Gene_locus related to this paper: picpg-c4r5w7 , picpg-c4r272 |
| Gene_locus | picpg-c4r5w7 picpg-c4r272 |
Prabha L, Govindappa N, Adhikary L, Melarkode R, Sastry K (2009)
Identification of the dipeptidyl aminopeptidase responsible for N-terminal clipping of recombinant Exendin-4 precursor expressed in Pichia pastoris
Protein Expr Purif
64 :155
Prabha L, Govindappa N, Adhikary L, Melarkode R, Sastry K (2009)
Protein Expr Purif
64 :155