Title : Hydroxynitrile lyase engineering for promiscuous asymmetric Henry reaction with enhanced conversion, enantioselectivity and catalytic efficiency - Priya_2023_Chem.Commun.(Camb)_59_12274 |
Author(s) : Priya BV , Rao DHS , Chatterjee A , Padhi SK |
Ref : Chem Commun (Camb) , 59 :12274 , 2023 |
Abstract :
Arabidopsis thaliana hydroxynitrile lyase (AtHNL) engineering has uncovered variants that showed up to 12-fold improved catalytic efficiency than the wild-type towards asymmetric Henry reaction. The AtHNL variants have displayed excellent enantioselectivity, up to >99%, and higher conversion in the synthesis of 13 different (R)-beta-nitroalcohols from their corresponding aldehydes. Using cell lysates of Y14M/F179W, we demonstrated a preparative scale synthesis of (R)-1-(4-methoxyphenyl)-2-nitroethanol, a tembamide chiral intermediate, in >99% ee and 52% yield. |
PubMedSearch : Priya_2023_Chem.Commun.(Camb)_59_12274 |
PubMedID: 37750925 |
Gene_locus related to this paper: arath-HNL |
Gene_locus | arath-HNL |
Priya BV, Rao DHS, Chatterjee A, Padhi SK (2023)
Hydroxynitrile lyase engineering for promiscuous asymmetric Henry reaction with enhanced conversion, enantioselectivity and catalytic efficiency
Chem Commun (Camb)
59 :12274
Priya BV, Rao DHS, Chatterjee A, Padhi SK (2023)
Chem Commun (Camb)
59 :12274