Priya_2023_Chem.Commun.(Camb)_59_12274

Reference

Title : Hydroxynitrile lyase engineering for promiscuous asymmetric Henry reaction with enhanced conversion, enantioselectivity and catalytic efficiency - Priya_2023_Chem.Commun.(Camb)_59_12274
Author(s) : Priya BV , Rao DHS , Chatterjee A , Padhi SK
Ref : Chem Commun (Camb) , 59 :12274 , 2023
Abstract :

Arabidopsis thaliana hydroxynitrile lyase (AtHNL) engineering has uncovered variants that showed up to 12-fold improved catalytic efficiency than the wild-type towards asymmetric Henry reaction. The AtHNL variants have displayed excellent enantioselectivity, up to >99%, and higher conversion in the synthesis of 13 different (R)-beta-nitroalcohols from their corresponding aldehydes. Using cell lysates of Y14M/F179W, we demonstrated a preparative scale synthesis of (R)-1-(4-methoxyphenyl)-2-nitroethanol, a tembamide chiral intermediate, in >99% ee and 52% yield.

PubMedSearch : Priya_2023_Chem.Commun.(Camb)_59_12274
PubMedID: 37750925
Gene_locus related to this paper: arath-HNL

Related information

Gene_locus arath-HNL

Citations formats

Priya BV, Rao DHS, Chatterjee A, Padhi SK (2023)
Hydroxynitrile lyase engineering for promiscuous asymmetric Henry reaction with enhanced conversion, enantioselectivity and catalytic efficiency
Chem Commun (Camb) 59 :12274

Priya BV, Rao DHS, Chatterjee A, Padhi SK (2023)
Chem Commun (Camb) 59 :12274