Qian_2016_Oncol.Rep_35_1273

Reference

Title : FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter - Qian_2016_Oncol.Rep_35_1273
Author(s) : Qian K , Zhang J , Lu J , Liu W , Yao X , Chen Q , Lu S , Xiang G , Liu H
Ref : Oncol Rep , 35 :1273 , 2016
Abstract :

In our previous study, low expression of FAM172A protein was found in colon cancer tissues. This research was planned to explore the functions of FAM172A gene and examine the mechanisms of its transcriptional regulation. Firstly, flow cytometry showed that FAM172A inhibited proliferation and promoted apoptosis and differentiation of colon cancer cells. Then through continuous truncation, we identified the minimal functional promoter region of FAM172A. Subsequently, we found that STAT1, as a transcription factor, could bind to the minimal FAM172A promoter, as evaluated using Chromatin immunoprecipitation (ChIP) and Electrophoreticmobility shift assay (EMSA). The results of Western blot analysis and qRT-PCR indicated that STAT1 was able to upregulate the expression of FAM172A. Our results showed that FAM172A could suppress proliferation of colon cancer cells, and STAT1 could bind to the minimum promoter region of FAM172A and upregulated the expression of FAM172A. These results may provide advanced insights into the functions of FAM172A and its regulatory mechanisms.

PubMedSearch : Qian_2016_Oncol.Rep_35_1273
PubMedID: 26676844
Gene_locus related to this paper: human-f172a

Related information

Gene_locus human-f172a
Family Arb2_FAM172A

Citations formats

Qian K, Zhang J, Lu J, Liu W, Yao X, Chen Q, Lu S, Xiang G, Liu H (2016)
FAM172A modulates apoptosis and proliferation of colon cancer cells via STAT1 binding to its promoter
Oncol Rep 35 :1273

Qian K, Zhang J, Lu J, Liu W, Yao X, Chen Q, Lu S, Xiang G, Liu H (2016)
Oncol Rep 35 :1273