Rabus_2005_Arch.Microbiol_183_27

Reference

Title : The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1 - Rabus_2005_Arch.Microbiol_183_27
Author(s) : Rabus R , Kube M , Heider J , Beck A , Heitmann K , Widdel F , Reinhardt R
Ref : Arch Microbiol , 183 :27 , 2005
Abstract :

Recent research on microbial degradation of aromatic and other refractory compounds in anoxic waters and soils has revealed that nitrate-reducing bacteria belonging to the Betaproteobacteria contribute substantially to this process. Here we present the first complete genome of a metabolically versatile representative, strain EbN1, which metabolizes various aromatic compounds, including hydrocarbons. A circular chromosome (4.3 Mb) and two plasmids (0.21 and 0.22 Mb) encode 4603 predicted proteins. Ten anaerobic and four aerobic aromatic degradation pathways were recognized, with the encoding genes mostly forming clusters. The presence of paralogous gene clusters (e.g., for anaerobic phenylacetate oxidation), high sequence similarities to orthologs from other strains (e.g., for anaerobic phenol metabolism) and frequent mobile genetic elements (e.g., more than 200 genes for transposases) suggest high genome plasticity and extensive lateral gene transfer during metabolic evolution of strain EbN1. Metabolic versatility is also reflected by the presence of multiple respiratory complexes. A large number of regulators, including more than 30 two-component and several FNR-type regulators, indicate a finely tuned regulatory network able to respond to the fluctuating availability of organic substrates and electron acceptors in the environment. The absence of genes required for nitrogen fixation and specific interaction with plants separates strain EbN1 ecophysiologically from the closely related nitrogen-fixing plant symbionts of the Azoarcus cluster. Supplementary material on sequence and annotation are provided at the Web page http://www.micro-genomes.mpg.de/ebn1/.

PubMedSearch : Rabus_2005_Arch.Microbiol_183_27
PubMedID: 15551059
Gene_locus related to this paper: aroae-metx , aroae-q5nz38 , aroae-q5nzf6 , aroae-q5nzt3 , aroae-q5nzz6 , aroae-q5p0p4 , aroae-q5p0w7 , aroae-q5p1d0 , aroae-q5p1l8 , aroae-q5p3d9 , aroae-q5p3m7 , aroae-q5p3t3 , aroae-q5p4k6 , aroae-q5p4m0 , aroae-q5p4u0 , aroae-q5p6k3 , aroae-q5p8f6 , aroae-q5p072 , aroae-q5p115 , aroae-q5p962 , aroae-q5p966 , aroae-q5p2a5 , aroae-q5p2a4

Related information

Gene_locus aroae-metx    aroae-q5nz38    aroae-q5nzf6    aroae-q5nzt3    aroae-q5nzz6    aroae-q5p0p4    aroae-q5p0w7    aroae-q5p1d0    aroae-q5p1l8    aroae-q5p3d9    aroae-q5p3m7    aroae-q5p3t3    aroae-q5p4k6    aroae-q5p4m0    aroae-q5p4u0    aroae-q5p6k3    aroae-q5p8f6    aroae-q5p072    aroae-q5p115    aroae-q5p962    aroae-q5p966    aroae-q5p2a5    aroae-q5p2a4

Citations formats

Rabus R, Kube M, Heider J, Beck A, Heitmann K, Widdel F, Reinhardt R (2005)
The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1
Arch Microbiol 183 :27

Rabus R, Kube M, Heider J, Beck A, Heitmann K, Widdel F, Reinhardt R (2005)
Arch Microbiol 183 :27