| Title : Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus - Raj_2014_J.Virol_88_1834 |
| Author(s) : Raj VS , Smits SL , Provacia LB , van den Brand JM , Wiersma L , Ouwendijk WJ , Bestebroer TM , Spronken MI , van Amerongen G , Rottier PJ , Fouchier RA , Bosch BJ , Osterhaus AD , Haagmans BL |
| Ref : J Virol , 88 :1834 , 2014 |
|
Abstract :
Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection. |
| PubMedSearch : Raj_2014_J.Virol_88_1834 |
| PubMedID: 24257613 |
Raj VS, Smits SL, Provacia LB, van den Brand JM, Wiersma L, Ouwendijk WJ, Bestebroer TM, Spronken MI, van Amerongen G, Rottier PJ, Fouchier RA, Bosch BJ, Osterhaus AD, Haagmans BL (2014)
Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus
J Virol
88 :1834
Raj VS, Smits SL, Provacia LB, van den Brand JM, Wiersma L, Ouwendijk WJ, Bestebroer TM, Spronken MI, van Amerongen G, Rottier PJ, Fouchier RA, Bosch BJ, Osterhaus AD, Haagmans BL (2014)
J Virol
88 :1834