Schulz_1998_J.Neurochem_71_853

Reference

Title : D alpha3, a new functional alpha subunit of nicotinic acetylcholine receptors from Drosophila - Schulz_1998_J.Neurochem_71_853
Author(s) : Schulz R , Sawruk E , Mulhardt C , Bertrand S , Baumann A , Phannavong B , Betz H , Bertrand D , Gundelfinger ED , Schmitt B
Ref : Journal of Neurochemistry , 71 :853 , 1998
Abstract :

Nicotinic acetylcholine (ACh) receptors (nAChRs) are important excitatory neurotransmitter receptors in the insect CNS. We have isolated and characterized the gene and the cDNA of a new nAChR subunit from Drosophila. The predicted mature nAChR protein consists of 773 amino acid residues and has the structural features of an ACh-binding alpha subunit. It was therefore named D alpha3, for Drosophila alpha-subunit 3. The d alpha3 gene maps to the X chromosome at position 7E. The properties of the D alpha3 protein were assessed by expression in Xenopus oocytes. D alpha3 did not form functional receptors on its own or in combination with any Drosophila beta-type nAChR subunit. Nondesensitizing ACh-evoked inward currents were observed when D alpha3 was coexpressed with the chick beta2 subunit. Half-maximal responses were at approximately 0.15 microM ACh with a Hill coefficient of approximately 1.5. The snake venom component alpha-bungarotoxin (100 nM) efficiently but reversibly blocked D alpha3/beta2 receptors, suggesting that D alpha3 may be a component of one of the previously described two classes of toxin binding sites in the Drosophila CNS.

PubMedSearch : Schulz_1998_J.Neurochem_71_853
PubMedID: 9681478

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Citations formats

Schulz R, Sawruk E, Mulhardt C, Bertrand S, Baumann A, Phannavong B, Betz H, Bertrand D, Gundelfinger ED, Schmitt B (1998)
D alpha3, a new functional alpha subunit of nicotinic acetylcholine receptors from Drosophila
Journal of Neurochemistry 71 :853

Schulz R, Sawruk E, Mulhardt C, Bertrand S, Baumann A, Phannavong B, Betz H, Bertrand D, Gundelfinger ED, Schmitt B (1998)
Journal of Neurochemistry 71 :853