Title : Myogenesis on microcarrier cultures - Shainberg_1983_Cell.Biol.Int.Rep_7_727 |
Author(s) : Shainberg A , Isac A , Reuveny S , Mizrahi A , Shahar A |
Ref : Cell Biology International Report , 7 :727 , 1983 |
Abstract :
The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle. |
PubMedSearch : Shainberg_1983_Cell.Biol.Int.Rep_7_727 |
PubMedID: 6627408 |
Shainberg A, Isac A, Reuveny S, Mizrahi A, Shahar A (1983)
Myogenesis on microcarrier cultures
Cell Biology International Report
7 :727
Shainberg A, Isac A, Reuveny S, Mizrahi A, Shahar A (1983)
Cell Biology International Report
7 :727