| Title : Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01) - Shen_2012_Biotechnol.Lett_34_2087 |
| Author(s) : Shen Y , Huang H , Zhu L , Luo M , Chen D |
| Ref : Biotechnol Lett , 34 :2087 , 2012 |
|
Abstract :
ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial for LW01 biosynthesis. ECO-TE I, the gene encoding type I thioesterase from LW01 polyketide synthases, cannot complement ECO-orf27 deficient mutant distinguishing ECO-orf27 from type I thioesterase gene. Type II thioesterase gene pikAV from Streptomyces venezuelae could complement ECO-orf27 in A. orientalis indicating that the two genes are equivalent in their function. Overexpression of ECO-orf27 resulted in a 20 % increase in LW01 production providing an alternative approach for yield improvement. |
| PubMedSearch : Shen_2012_Biotechnol.Lett_34_2087 |
| PubMedID: 22850790 |
Shen Y, Huang H, Zhu L, Luo M, Chen D (2012)
Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01)
Biotechnol Lett
34 :2087
Shen Y, Huang H, Zhu L, Luo M, Chen D (2012)
Biotechnol Lett
34 :2087