| Title : Rs15285, a functional polymorphism located in lipoprotein lipase, predicts the risk and prognosis of gastric cancer - Shen_2023_Appl.Microbiol.Biotechnol__ |
| Author(s) : Shen K , Zhou X , Hu L , Xiao J , Cheng Q , Wang Y , Liu K , Fan H , Xu Z , Yang L |
| Ref : Applied Microbiology & Biotechnology , : , 2023 |
| Abstract : Lipoprotein lipase (LPL), a crucial gene in lipid metabolism, has a significant role in the progression of malignant tumors. The purpose of this research was to investigate the impact of rs15285 found in the LPL gene's 3'UTR region on the risk, biological behavior, and gastric cancer (GC) prognosis as well as to examine its potential function. Genotyping of rs15285 in 888 GC cases and 874 controls was conducted by SNaPshot technology. We used bioinformatics analysis and in vitro experiments to study the role of rs15285. First, this study revealed for the first time that polymorphism rs15285 increases the risk of GC (OR = 1.48, 95%CI = 1.16-1.89, P = 0.002). Although no relationship was found between rs12585 and the pathological features of GC, the prognosis of individuals with the rs12585 TT genotype was poorer than that of patients with the CC or CC+CT genotype (HR = 2.39 for TT vs. CC, P = 0.025; HR = 2.38 for TT vs. CC+CT, P = 0.025). In addition, bioinformatics analysis showed rs12585 may affect the binding of miRNAs to LPL, resulting in an increase of LPL expression to promote cancer progression. Ultimately, in vitro tests revealed that the rs15285 T allele increased LPL expression on the mRNA as well as the protein levels, promoting GC cell proliferation, invasion, and metastasis. The LPL rs12528 TT genotype increased the risk of GC and predicted a poor prognosis. Mechanistically, the rs15285 T allele could improve the expression of LPL, and thus promotes the malignant phenotype of GC. Therefore, our study may provide new biological predictors and a theoretical basis for the prognosis and customized therapy of stomach cancer patients. KEY POINTS: Rs15285 polymorphism is a risk factor for GC. Rs12585 TT genotype predicts a bad outcome in GC individuals. Rs15285 T allele enhances GC cells malignant biological behavior. |
| ESTHER : Shen_2023_Appl.Microbiol.Biotechnol__ |
| PubMedSearch : Shen_2023_Appl.Microbiol.Biotechnol__ |
| PubMedID: 37036527 |
| Gene_locus related to this paper: human-LPL |
| Gene_locus related to this paper: human-LPL |
Shen K, Zhou X, Hu L, Xiao J, Cheng Q, Wang Y, Liu K, Fan H, Xu Z, Yang L (2023)
Rs15285, a functional polymorphism located in lipoprotein lipase, predicts the risk and prognosis of gastric cancer
Applied Microbiology & Biotechnology
:
Shen K, Zhou X, Hu L, Xiao J, Cheng Q, Wang Y, Liu K, Fan H, Xu Z, Yang L (2023)
Applied Microbiology & Biotechnology
: