Title : Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study - Shinozaki_2013_Appl.Microbiol.Biotechnol_97_8591 |
Author(s) : Shinozaki Y , Kikkawa Y , Sato S , Fukuoka T , Watanabe T , Yoshida S , Nakajima-kambe T , Kitamoto HK |
Ref : Applied Microbiology & Biotechnology , 97 :8591 , 2013 |
Abstract :
Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica JCM10317 (PaE) was analyzed by surface plasmon resonance (SPR). The adsorption of PaE and the degradation rate for polyester films were quantitatively monitored by a positive and negative SPR signal shifts, respectively. The decrease in SPR signal and the erosion depth of amorphous poly(L-lactide) (a-PLLA) film measured by atomic force microscopy (AFM) had a linear relationship, and the weight loss was estimated from the AFM data combined with a density of a-PLLA film. Furthermore, SPR sensorgrams for various polyester films showed that degradation rate of poly(sigma-caprolactone) and poly(butylene succinate-co-adipate) which contain C6 units was higher than that of other polyesters such as poly(butylene succinate) and a-PLLA. These results suggest that C6 is the preferred chain length as substrates for PaE. |
PubMedSearch : Shinozaki_2013_Appl.Microbiol.Biotechnol_97_8591 |
PubMedID: 23339012 |
Gene_locus related to this paper: psea2-s6bc01 |
Substrate | Polylactic-acid |
Gene_locus | psea2-s6bc01 |
Shinozaki Y, Kikkawa Y, Sato S, Fukuoka T, Watanabe T, Yoshida S, Nakajima-kambe T, Kitamoto HK (2013)
Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study
Applied Microbiology & Biotechnology
97 :8591
Shinozaki Y, Kikkawa Y, Sato S, Fukuoka T, Watanabe T, Yoshida S, Nakajima-kambe T, Kitamoto HK (2013)
Applied Microbiology & Biotechnology
97 :8591