Shu_2023_Enzyme.Microb.Technol_169_110266

Reference

Title : Transesterification of phosphatidylcholine with DHA-rich algal oil using immobilized Candida antarctica lipase B to produce DHA-phosphatidylcholine - Shu_2023_Enzyme.Microb.Technol_169_110266
Author(s) : Shu L , Zheng X , Qi S , Lin S , Lu Y , Yao C , Ling X
Ref : Enzyme Microb Technol , 169 :110266 , 2023
Abstract :

Docosahexaenoic acid (DHA) enriched with phospholipids (PLs) (DHA-PLs) is a type of structured PL with good physicochemical and nutritional properties. Compared to PLs and DHA, DHA-PLs has higher bioavailability and structural stability and many nutritional benefits. To improve the enzymatic synthesis of DHA-PLs, this study investigated the preparation of phosphatidylcholine (PC) enriched with DHA (DHA-PC) via enzymatic transesterification of algal oil, which is rich in DHA-triglycerides, using immobilized Candida antarctica lipase B (CALB). The optimized reaction system incorporated 31.2% DHA into the acyl chain of PC and converted 43.6% PC to DHA-PC within 72 h at 50 degreesC, 1:8 PC: algal oil mass ratio, 25% enzyme load (based on total substrate mass), and 0.02 g/mL molecular sieve concentration. Consequently, the side reactions of PC hydrolysis were effectively suppressed and products with high PC content (74.8%) were produced. Molecular structure analysis showed that exogenous DHA was specifically incorporated into the sn-1 site of the PC by immobilized CALB. Furthermore, the evaluation of reusability with eight cycles showed that the immobilized CALB had good operational stability in the present reaction system. Collectively, this study demonstrated the applicability of immobilized CALB as a biocatalyst for synthesizing DHA-PC and provided an improved enzyme-catalyzed method for future DHA-PL synthesis.

PubMedSearch : Shu_2023_Enzyme.Microb.Technol_169_110266
PubMedID: 37311283

Related information

Citations formats

Shu L, Zheng X, Qi S, Lin S, Lu Y, Yao C, Ling X (2023)
Transesterification of phosphatidylcholine with DHA-rich algal oil using immobilized Candida antarctica lipase B to produce DHA-phosphatidylcholine
Enzyme Microb Technol 169 :110266

Shu L, Zheng X, Qi S, Lin S, Lu Y, Yao C, Ling X (2023)
Enzyme Microb Technol 169 :110266