Shui_1998_J.Physiol_507 ( Pt 2)_325

Reference

Title : Role of receptor kinase in short-term desensitization of cardiac muscarinic K+ channels expressed in Chinese hamster ovary cells - Shui_1998_J.Physiol_507 ( Pt 2)_325
Author(s) : Shui Z , Khan IA , Tsuga H , Haga T , Boyett MR
Ref : Journal of Physiology , 507 ( Pt 2) :325 , 1998
Abstract :

1. The cardiac muscarinic receptor-K+ channel system was reconstructed in Chinese hamster ovary (CHO) cells by transfecting the cells with the various components of the system. The activity of the muscarinic K+ channel was measured with the cell-attached configuration of the patch clamp technique. 2. In CHO cells transfected with the channel (Kir3.1/Kir3.4), receptor (hm2) and receptor kinase (GRK2), on exposure to agonist, there was a decline in channel activity as a result of desensitization, similar to that in atrial cells. 3. Whereas the desensitization was almost abolished by not transfecting with the receptor kinase or by transfecting with a mutant receptor lacking phosphorylation sites, it was only reduced (by approximately 39%) by transfecting with a mutant receptor kinase with little/kinase activity. 4. These results suggest that the receptor kinase is responsible for desensitization of the muscarinic K+ channel and that this involves phosphorylation-dependent and -independent mechanisms.

PubMedSearch : Shui_1998_J.Physiol_507 ( Pt 2)_325
PubMedID: 9518696

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Citations formats

Shui Z, Khan IA, Tsuga H, Haga T, Boyett MR (1998)
Role of receptor kinase in short-term desensitization of cardiac muscarinic K+ channels expressed in Chinese hamster ovary cells
Journal of Physiology 507 ( Pt 2) :325

Shui Z, Khan IA, Tsuga H, Haga T, Boyett MR (1998)
Journal of Physiology 507 ( Pt 2) :325