| Title : [Bacterial expression of water-soluble domain of Lynx1, endogenic neuromodulator of human nicotinic acetylcholine receptors] - Shulepko_2011_Bioorg.Khim_37_609 |
| Author(s) : Shulepko MA , Liukmanova EN , Kasheverov IE , Dolgikh DA , Tsetlin VI , Kirpichnikov MP |
| Ref : Bioorganicheskaia Khimiia , 37 :609 , 2011 |
|
Abstract :
Lynx1 expresses in the central nervous system and plays important role in a regulation of nicotinic acetylcholine receptors. Successful milligram-quantitive expression of ws-Lynx1 was achieved only in the case of its production in the form of cytoplasm inclusion bodies. Different conditions of ws-Lynx1 refolding for yield optimization were performed. The obtained recombinant protein was characterized by means of mass spectrometry and CD spectroscopy. The binding experiments on the nAChRs from Torpedo californica membranes revealed that ws-Lynxl is biologically active and blocks muscle nAChR with IC50-20-30 microM. |
| PubMedSearch : Shulepko_2011_Bioorg.Khim_37_609 |
| PubMedID: 22332355 |
Shulepko MA, Liukmanova EN, Kasheverov IE, Dolgikh DA, Tsetlin VI, Kirpichnikov MP (2011)
[Bacterial expression of water-soluble domain of Lynx1, endogenic neuromodulator of human nicotinic acetylcholine receptors]
Bioorganicheskaia Khimiia
37 :609
Shulepko MA, Liukmanova EN, Kasheverov IE, Dolgikh DA, Tsetlin VI, Kirpichnikov MP (2011)
Bioorganicheskaia Khimiia
37 :609