Son_2020_Enzyme.Microb.Technol_141_109656

Reference

Title : Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis - Son_2020_Enzyme.Microb.Technol_141_109656
Author(s) : Son HF , Joo S , Seo H , Sagong HY , Lee SH , Hong H , Kim KJ
Ref : Enzyme Microb Technol , 141 :109656 , 2020
Abstract :

Poly(ethylene terephthalate) (PET), a widely used plastic around the world, causes various environmental and health problems. Several groups have been extensively conducting research to solve these problems through enzymatic degradation of PET at high temperatures around 70 degC. Recently, Ideonella sakaiensis, a bacterium that degrades PET at mild temperatures, has been newly identified, and further protein engineering studies on the PET degrading enzyme from the organism (IsPETase) have also been conducted to overcome the low thermal stability of the enzyme. In this study, we performed structural bioinformatics-based protein engineering of IsPETase to optimize the substrate binding site of the enzyme and developed two variants, IsPETase(S242T) and IsPETase(N246D), with higher enzymatic activity at both 25 and 37 degC compared with IsPETase(WT). We also developed the IsPETase(S121E/D186H/S242T/N246D) variant by integrating the S242T and N246D mutations into the previously reported IsPETase(S121E/D186H/R208A) variant. At the 37 degC incubation, the quadruple variant maintained the PET degradation activity for 20 days, unlike IsPETase(WT) that lost its activity within a day. Consequently, this study exhibited 58-fold increase in the activity compared with IsPETase(WT).

PubMedSearch : Son_2020_Enzyme.Microb.Technol_141_109656
PubMedID: 33051015
Gene_locus related to this paper: idesa-mheth

Related information

Gene_locus idesa-mheth
Family idesa-mheth    Tannase
Structure idesa-mheth    Tannase    6KUO

Citations formats

Son HF, Joo S, Seo H, Sagong HY, Lee SH, Hong H, Kim KJ (2020)
Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis
Enzyme Microb Technol 141 :109656

Son HF, Joo S, Seo H, Sagong HY, Lee SH, Hong H, Kim KJ (2020)
Enzyme Microb Technol 141 :109656