idesa-mheth

Ideonella sakaiensis (strain 201-F6) (Piscinibacter sakaiensis) Mono(2-hydroxyethyl) terephthalate hydrolase MHETase, MHET hydrolase, Mle046, exo-PETase IsMHETase

Comment

Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of mono(2-hydroxyethyl) terephthalate into its two environmentally benign monomers, terephthalate and ethylene glycol. Does not show activity against PET (which is hydrolysed by idesa-peth) (but PET pentamer is a substrate), bis(hydroxyethyl) terephthalate (BHET), pNP-aliphatic esters or typical aromatic ester compounds catalyzed by the tannase family enzymes, such as ethyl gallate and ethyl ferulate. However some mutants of idesa-mheth can hydrolyze BHET ( Palm et al.<\/A>)

Relationship

Family : Tannase

Block : X

Position in NCBI Life Tree : Ideonella sakaiensis

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Proteobacteria N E > Betaproteobacteria N E > Burkholderiales N E > unclassified Burkholderiales N E > Burkholderiales Genera incertae sedis N E > Ideonella N E > Ideonella sakaiensis N E

Database

Sequence

Peptide

MQTTVTTMLL ASVALAACAG GGSTPLPLPQ QQPPQQEPPP PPVPLASRAA CEALKDGNGD MVWPNAATVV EVAAWRDAAP ATASAAALPE HCEVSGAIAK RTGIDGYPYE IKFRLRMPAE WNGRFFMEGG SGTNGSLSAA TGSIGGGQIA SALSRNFATI ATDGGHDNAV NDNPDALGTV AFGLDPQARL DMGYNSYDQV TQAGKAAVAR FYGRAADKSY FIGCSEGGRE GMMLSQRFPS HYDGIVAGAP GYQLPKAGIS GAWTTQSLAP AAVGLDAQGV PLINKSFSDA DLHLLSQAIL GTCDALDGLA DGIVDNYRAC QAAFDPATAA NPANGQALQC VGAKTADCLS PVQVTAIKRA MAGPVNSAGT PLYNRWAWDA GMSGLSGTTY NQGWRSWWLG SFNSSANNAQ RVSGFSARSW LVDFATPPEP MPMTQVAARM MKFDFDIDPL KIWATSGQFT QSSMDWHGAT STDLAAFRDR GGKMILYHGM SDAAFSALDT ADYYERLGAA MPGAAGFARL FLVPGMNHCS GGPGTDRFDM LTPLVAWVER GEAPDQISAW SGTPGYFGVA ARTRPLCPYP QIARYKGSGD INTEANFACA APP

References (18)

Title : Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol - Loll-Krippleber_2022_Microb.Cell.Fact_21_280
Author(s) : Loll-Krippleber R , Sajtovich VA , Ferguson MW , Ho B , Burns AR , Payliss BJ , Bellissimo J , Peters S , Roy PJ , Wyatt HDM , Brown GW
Ref : Microb Cell Fact , 21 :280 , 2022
PubMedID: 36587193
Gene_locus related to this paper: idesa-mheth

Title : Rapid depolymerization of poly(ethylene terephthalate) thin films by a dual-enzyme system and its impact on material properties - Tarazona_2022_Chem.Catal_2_3573
Author(s) : Tarazona NA , Wei R , Brott S , Pfaff L , Bornscheuer UT , Lendlein A , Machatschek R
Ref : Chem Catal , 2 :3573 , 2022
PubMedID: 37350932
Gene_locus related to this paper: idesa-mheth , idesa-peth

Title : Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET - Magalhaes_2021_Int.J.Mol.Sci_22_11257
Author(s) : Magalhaes RP , Cunha JM , Sousa SF
Ref : Int J Mol Sci , 22 :11257 , 2021
PubMedID: 34681915
Gene_locus related to this paper: 9entr-EsEstB , 9bact-a0a2h5z9r5 , 9psed-peh , 9actn-h6wx58 , idesa-mheth , idesa-peth , thecd-d1a2h1 , humin-cut , 9acto-d4q9n1 , 9acto-f7ix06 , 9bact-g9by57 , bacsu-lip , bacsu-pnbae , canar-LipB , thela-m4tp71 , psemy-a4y035 , thefu-1831 , thefu-q6a0i4 , strsw-c9zcr8 , fusox-CUT , sacvd-c7mve8

Title : Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis - Graf_2021_Methods.Enzymol_648_337
Author(s) : Graf LG , Michels EAP , Yew Y , Liu W , Palm GJ , Weber G
Ref : Methods Enzymol , 648 :337 , 2021
PubMedID: 33579411
Gene_locus related to this paper: idesa-mheth , idesa-peth

Title : Reaction Mechanism of MHETase, a PET Degrading Enzyme - Pinto_2021_ACS.Catal_11_10416
Author(s) : Pinto AV , Ferreira P , Neves RPP , Fernandes PA , Ramos MJ , Magalhaes AL
Ref : ACS Catal , 11 :10416 , 2021
PubMedID:
Gene_locus related to this paper: idesa-mheth

Title : Development of a targeted gene disruption system in the PET-degrading bacterium Ideonella sakaiensis and its applications to PETase and MHETase genes - Hachisuka_2021_Appl.Environ.Microbiol__AEM0002021
Author(s) : Hachisuka SI , Nishii T , Yoshida S
Ref : Applied Environmental Microbiology , :AEM0002021 , 2021
PubMedID: 34260304
Gene_locus related to this paper: idesa-mheth , idesa-peth

Title : IsPETase represents a novel biocatalyst for poly (ethylene terephthalate) (PET) hydrolysis - Kan_2021_Chembiochem__
Author(s) : Kan Y , He L , Luo Y , Bao R
Ref : Chembiochem , : , 2021
PubMedID: 33434375
Gene_locus related to this paper: idesa-mheth

Title : Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to beta-ketoadipic acid by Pseudomonas putida KT2440 - Werner_2021_Metab.Eng_67_250
Author(s) : Werner AZ , Clare R , Mand TD , Pardo I , Ramirez KJ , Haugen SJ , Bratti F , Dexter GN , Elmore JR , Huenemann JD , Peabody GLt , Johnson CW , Rorrer NA , Salvachua D , Guss AM , Beckham GT
Ref : Metab Eng , 67 :250 , 2021
PubMedID: 34265401
Gene_locus related to this paper: idesa-mheth , idesa-peth

Title : Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization - Yoshida_2021_Methods.Enzymol_648_187
Author(s) : Yoshida S , Hiraga K , Taniguchi I , Oda K
Ref : Methods Enzymol , 648 :187 , 2021
PubMedID: 33579403
Gene_locus related to this paper: idesa-mheth , idesa-peth

Title : Mle046 Is a Marine Mesophilic MHETase-Like Enzyme - Meyer-Cifuentes_2021_Front.Microbiol_12_693985
Author(s) : Meyer-Cifuentes IE , Ozturk B
Ref : Front Microbiol , 12 :693985 , 2021
PubMedID: 34381429
Gene_locus related to this paper: idesa-mheth

Title : Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium - Meyer-Cifuentes_2020_Nat.Commun_11_5790
Author(s) : Meyer-Cifuentes IE , Werner J , Jehmlich N , Will SE , Neumann-Schaal M , zturk B
Ref : Nat Commun , 11 :5790 , 2020
PubMedID: 33188179
Gene_locus related to this paper: 9zzzz-Ple628 , 9zzzz-Ple629 , idesa-mheth

Title : Decomposition of the PET Film by MHETase Using Exo-PETase Function - Sagong_2020_ACS.Catal_10_48050
Author(s) : Sagong HY , Seo H , Kim T , Son H , Joo S , Lee S , Kim S , Woo JS , Hwang S , Kim KJ
Ref : ACS Catal , 10 :4805 , 2020
PubMedID:
Gene_locus related to this paper: idesa-mheth

Title : Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis - Son_2020_Enzyme.Microb.Technol_141_109656
Author(s) : Son HF , Joo S , Seo H , Sagong HY , Lee SH , Hong H , Kim KJ
Ref : Enzyme Microb Technol , 141 :109656 , 2020
PubMedID: 33051015
Gene_locus related to this paper: idesa-mheth

Title : Characterization and engineering of a two-enzyme system for plastics depolymerization - Knott_2020_Proc.Natl.Acad.Sci.U.S.A_117_25476
Author(s) : Knott BC , Erickson E , Allen MD , Gado JE , Graham R , Kearns FL , Pardo I , Topuzlu E , Anderson JJ , Austin HP , Dominick G , Johnson CW , Rorrer NA , Szostkiewicz CJ , Copie V , Payne CM , Woodcock HL , Donohoe BS , Beckham GT , McGeehan JE
Ref : Proc Natl Acad Sci U S A , 117 :25476 , 2020
PubMedID: 32989159
Gene_locus related to this paper: idesa-mheth

Title : Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate - Palm_2019_Nat.Commun_10_1717
Author(s) : Palm GJ , Reisky L , Bottcher D , Muller H , Michels EAP , Walczak MC , Berndt L , Weiss MS , Bornscheuer UT , Weber G
Ref : Nat Commun , 10 :1717 , 2019
PubMedID: 30979881
Gene_locus related to this paper: idesa-mheth

Title : Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation - Son_2019_ACS.Catal_9_3519
Author(s) : Son HF , Cho IJ , Joo S , Seo H , Sagong HY , Choi SY , Lee SY , Kim KJ
Ref : ACS Catal , 9 :3519 , 2019
PubMedID:
Gene_locus related to this paper: idesa-mheth

Title : Production of extracellular PETase from Ideonella sakaiensis using sec-dependent signal peptides in E. coli - Seo_2019_Biochem.Biophys.Res.Commun_508_250
Author(s) : Seo H , Kim S , Son HF , Sagong HY , Joo S , Kim KJ
Ref : Biochemical & Biophysical Research Communications , 508 :250 , 2019
PubMedID: 30477746
Gene_locus related to this paper: idesa-mheth

Title : A bacterium that degrades and assimilates poly(ethylene terephthalate) - Yoshida_2016_Science_351_1196
Author(s) : Yoshida S , Hiraga K , Takehana T , Taniguchi I , Yamaji H , Maeda Y , Toyohara K , Miyamoto K , Kimura Y , Oda K
Ref : Science , 351 :1196 , 2016
PubMedID: 26965627
Gene_locus related to this paper: idesa-mheth , idesa-peth