Son_2023_IUCrJ_10_220

Reference

Title : Structural and biochemical insights into PsEst3, a new GHSR-type esterase obtained from Paenibacillus sp. R4 - Son_2023_IUCrJ_10_220
Author(s) : Son J , Choi W , Kim H , Kim M , Lee JH , Shin SC , Kim HW
Ref : IUCrJ , 10 :220 , 2023
Abstract :

PsEst3, a psychrophilic esterase obtained from Paenibacillus sp. R4, which was isolated from the permafrost of Alaska, exhibits relatively high activity at low temperatures. Here, crystal structures of PsEst3 complexed with various ligands were generated and studied at atomic resolution, and biochemical studies were performed to analyze the structure-function relationship of PsEst3. Certain unique characteristics of PsEst3 distinct from those of other classes of lipases/esterases were identified. Firstly, PsEst3 contains a conserved GHSRA/G pentapeptide sequence in the GxSxG motif around the nucleophilic serine. Additionally, it contains a conserved HGFR/K consensus sequence in the oxyanion hole, which is distinct from that in other lipase/esterase families, as well as a specific domain composition (for example a helix-turn-helix motif) and a degenerative lid domain that exposes the active site to the solvent. Secondly, the electrostatic potential of the active site in PsEst3 is positive, which may cause unintended binding of negatively charged chemicals in the active site. Thirdly, the last residue of the oxyanion hole-forming sequence, Arg44, separates the active site from the solvent by sealing the acyl-binding pocket, suggesting that PsEst3 is an enzyme that is customized to sense an unidentified substrate that is distinct from those of classical lipases/esterases. Collectively, this evidence strongly suggests that PsEst3 belongs to a distinct family of esterases.

PubMedSearch : Son_2023_IUCrJ_10_220
PubMedID: 36862488
Gene_locus related to this paper: 9mico-PsEst3

Related information

Inhibitor Malonic-acid    PMSF
Gene_locus Malonic-acid    PMSF    9mico-PsEst3
Structure Malonic-acid    PMSF    9mico-PsEst3    7V8U    7V8V    7V8W    7V8X

Citations formats

Son J, Choi W, Kim H, Kim M, Lee JH, Shin SC, Kim HW (2023)
Structural and biochemical insights into PsEst3, a new GHSR-type esterase obtained from Paenibacillus sp. R4
IUCrJ 10 :220

Son J, Choi W, Kim H, Kim M, Lee JH, Shin SC, Kim HW (2023)
IUCrJ 10 :220