Stinear_2007_Genome.Res_17_192

Reference

Title : Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer - Stinear_2007_Genome.Res_17_192
Author(s) : Stinear TP , Seemann T , Pidot S , Frigui W , Reysset G , Garnier T , Meurice G , Simon D , Bouchier C , Ma L , Tichit M , Porter JL , Ryan J , Johnson PD , Davies JK , Jenkin GA , Small PL , Jones LM , Tekaia F , Laval F , Daffe M , Parkhill J , Cole ST
Ref : Genome Res , 17 :192 , 2007
Abstract :

Mycobacterium ulcerans is found in aquatic ecosystems and causes Buruli ulcer in humans, a neglected but devastating necrotic disease of subcutaneous tissue that is rampant throughout West and Central Africa. Here, we report the complete 5.8-Mb genome sequence of M. ulcerans and show that it comprises two circular replicons, a chromosome of 5632 kb and a virulence plasmid of 174 kb. The plasmid is required for production of the polyketide toxin mycolactone, which provokes necrosis. Comparisons with the recently completed 6.6-Mb genome of Mycobacterium marinum revealed >98% nucleotide sequence identity and genome-wide synteny. However, as well as the plasmid, M. ulcerans has accumulated 213 copies of the insertion sequence IS2404, 91 copies of IS2606, 771 pseudogenes, two bacteriophages, and multiple DNA deletions and rearrangements. These data indicate that M. ulcerans has recently evolved via lateral gene transfer and reductive evolution from the generalist, more rapid-growing environmental species M. marinum to become a niche-adapted specialist. Predictions based on genome inspection for the production of modified mycobacterial virulence factors, such as the highly abundant phthiodiolone lipids, were confirmed by structural analyses. Similarly, 11 protein-coding sequences identified as M. ulcerans-specific by comparative genomics were verified as such by PCR screening a diverse collection of 33 strains of M. ulcerans and M. marinum. This work offers significant insight into the biology and evolution of mycobacterial pathogens and is an important component of international efforts to counter Buruli ulcer.

PubMedSearch : Stinear_2007_Genome.Res_17_192
PubMedID: 17210928
Gene_locus related to this paper: mycmm-b2hds9 , mycmm-b2hg81 , mycmm-b2hgg2 , mycmm-b2hj55 , mycmm-b2hju3 , mycmm-b2hlr0 , mycmm-b2hlv2 , mycmm-b2hq96 , mycmm-b2hsm8 , mycmr-q5sdq4 , myctu-RV1683 , mycua-a0pkg7 , mycua-a0pki1 , mycua-a0pkn2 , mycua-a0pkn5 , mycua-a0pku2 , mycua-a0pl47 , mycua-a0plr3 , mycua-a0plu8 , mycua-a0ply4 , mycua-a0pm12 , mycua-a0pm14 , mycua-a0pme3 , mycua-a0pmj6 , mycua-a0pml9 , mycua-a0pmv0 , mycua-a0pmx9 , mycua-a0pn71 , mycua-a0png7 , mycua-a0png9 , mycua-a0pp56 , mycua-a0ppm6 , mycua-a0pqm2 , mycua-a0pqs2 , mycua-a0pr64 , mycua-a0pr98 , mycua-a0prq2 , mycua-a0prr7 , mycua-a0psb1 , mycua-a0psb4 , mycua-a0psi2 , mycua-a0psi3 , mycua-a0psu3 , mycua-a0pt08 , mycua-a0pt71 , mycua-a0pth6 , mycua-a0ptq0 , mycua-a0pu55 , mycua-a0pum4 , mycua-a0pv11 , mycua-a0pv36 , mycua-a0pv77 , mycua-a0pva4 , mycua-a0pwi8 , mycua-a0pwm4 , mycua-a0pwr6 , mycua-a0pwz5 , mycua-a0px52 , mycua-metx , mycua-a0pmc2 , mycua-a0pvg7 , mycua-a0pwz4 , mycmm-b2hqy3 , mycua-a0pmc3

Related information

Gene_locus mycmm-b2hds9    mycmm-b2hg81    mycmm-b2hgg2    mycmm-b2hj55    mycmm-b2hju3    mycmm-b2hlr0    mycmm-b2hlv2    mycmm-b2hq96    mycmm-b2hsm8    mycmr-q5sdq4    myctu-RV1683    mycua-a0pkg7    mycua-a0pki1    mycua-a0pkn2    mycua-a0pkn5    mycua-a0pku2    mycua-a0pl47    mycua-a0plr3    mycua-a0plu8    mycua-a0ply4    mycua-a0pm12    mycua-a0pm14    mycua-a0pme3    mycua-a0pmj6    mycua-a0pml9    mycua-a0pmv0    mycua-a0pmx9    mycua-a0pn71    mycua-a0png7    mycua-a0png9    mycua-a0pp56    mycua-a0ppm6    mycua-a0pqm2    mycua-a0pqs2    mycua-a0pr64    mycua-a0pr98    mycua-a0prq2    mycua-a0prr7    mycua-a0psb1    mycua-a0psb4    mycua-a0psi2    mycua-a0psi3    mycua-a0psu3    mycua-a0pt08    mycua-a0pt71    mycua-a0pth6    mycua-a0ptq0    mycua-a0pu55    mycua-a0pum4    mycua-a0pv11    mycua-a0pv36    mycua-a0pv77    mycua-a0pva4    mycua-a0pwi8    mycua-a0pwm4    mycua-a0pwr6    mycua-a0pwz5    mycua-a0px52    mycua-metx    mycua-a0pmc2    mycua-a0pvg7    mycua-a0pwz4    mycmm-b2hqy3    mycua-a0pmc3
Gene_locus_frgt mycua-a0pt22

Citations formats

Stinear TP, Seemann T, Pidot S, Frigui W, Reysset G, Garnier T, Meurice G, Simon D, Bouchier C, Ma L, Tichit M, Porter JL, Ryan J, Johnson PD, Davies JK, Jenkin GA, Small PL, Jones LM, Tekaia F, Laval F, Daffe M, Parkhill J, Cole ST (2007)
Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer
Genome Res 17 :192

Stinear TP, Seemann T, Pidot S, Frigui W, Reysset G, Garnier T, Meurice G, Simon D, Bouchier C, Ma L, Tichit M, Porter JL, Ryan J, Johnson PD, Davies JK, Jenkin GA, Small PL, Jones LM, Tekaia F, Laval F, Daffe M, Parkhill J, Cole ST (2007)
Genome Res 17 :192