| Title : Functional expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors precedes the development of cholinergic phenotype in embryonic rat septal cells in culture - Suzuki_2001_Neurosci.Lett_311_89 |
| Author(s) : Suzuki T , Matsugi T , Takagi R , Kanagawa M , Hirata M , Nakamura T , Kudo Y , Kawashima K |
| Ref : Neuroscience Letters , 311 :89 , 2001 |
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Abstract :
We examined the development of cholinergic neuronal functions and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) responses in cultured embryonic rat septal cells. Choline acetyltransferase activity was increased from 4 to 6 days in culture and reached a plateau at day 8. Acetylcholine release was increased from 6 to 8 days in culture. AMPA-induced increase in intracellular Ca(2+) level was observed at 3 days in culture and most of the AMPA-responsive cells coincided with high-K(+) responsive cells. These results suggest that cholinergic neurons develop their neuronal functions about 8 days under cultured conditions, and functional expression of AMPA receptors precedes the cholinergic functional development. |
| PubMedSearch : Suzuki_2001_Neurosci.Lett_311_89 |
| PubMedID: 11567785 |
Suzuki T, Matsugi T, Takagi R, Kanagawa M, Hirata M, Nakamura T, Kudo Y, Kawashima K (2001)
Functional expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors precedes the development of cholinergic phenotype in embryonic rat septal cells in culture
Neuroscience Letters
311 :89
Suzuki T, Matsugi T, Takagi R, Kanagawa M, Hirata M, Nakamura T, Kudo Y, Kawashima K (2001)
Neuroscience Letters
311 :89