| Title : Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12 - Tan_2015_Microbes.Environ_30_76 |
| Author(s) : Tan GY , Chen CL , Ge L , Li L , Tan SN , Wang JY |
| Ref : Microbes Environ , 30 :76 , 2015 |
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Abstract :
Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05+/-0.00 and 23.10+/-3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a genetically-distinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28+/-0.10 g L(-1) and PHA content of 32.49+/-2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6 ), 3-hydroxyoctanoate (C8 ), 3-hydroxydecanoate (C10 ), 3-hydroxydodecanoate (C12 ), and 3-hydroxytetradecanoate (C14 ) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA. |
| PubMedSearch : Tan_2015_Microbes.Environ_30_76 |
| PubMedID: 25740622 |
| Substrate | 3-hydroxydodecanoate |
Tan GY, Chen CL, Ge L, Li L, Tan SN, Wang JY (2015)
Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12
Microbes Environ
30 :76
Tan GY, Chen CL, Ge L, Li L, Tan SN, Wang JY (2015)
Microbes Environ
30 :76