| Title : Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1 - Tang_2018_Life.Sci_213_166 |
| Author(s) : Tang S , Lu C , Mo L , Wang X , Liang Z , Qin F , Liu Y , Huang H , Huang Y , Cai H , Xiao D , Guo S , Ouyang Y , Sun B , Li X |
| Ref : Life Sciences , 213 :166 , 2018 |
| Abstract : AIMS: Protein phosphatase methylesterase-1 (PME-1) is a serine hydrolase that catalyzes protein phosphatase 2A (PP2A) demethylation and negatively regulates its activity. PME-1 is compartmentalized within cells to precisely control the demethylation of PP2A. This study investigated the localization of PME-1 in human fibroblast cells (HDF) under oxidative stress. MAIN METHODS: Alkaline demethylation and peptide competition assays were applied to detect the methylation sensitivity of anti-PP2Ac. The localization of PME-1, leucine carboxyl methyltransferase 1 (LCMT1), demethylated-phosphorylated-PP2Ac (dem-p-PP2Ac) and total PP2Ac was determined by immunofluorescence analysis, and protein expression was measured by Western blot. A HEK293 cell line stably expressing constructed PME-1-EGFP was used to dynamically monitor the nuclear export of PME-1 under oxidative stress. KEY RESULTS: After hydrogen peroxide (H(2)O(2)) treatment, the protein expression of PME-1 remained unchanged, while PME-1 facilitated redistribution from the nucleus to the cytoplasm in HDF according to immunofluorescence analysis. In constructed HEK293 cells, the EGFP-tagged PME-1 was exported from the nucleus to the cytoplasm after H(2)O(2) treatment, and nuclear export was eliminated after leptomycin B additions. Our observation of dem-p-PP2Ac species relocation from the nucleus to the cytoplasm under oxidative stress is consistent with the redistribution patterns of PME-1. Antioxidant N-acetyl cysteine can reverse the nuclear to cytoplasmic ratio of PME-1 proteins and dem-p-PP2Ac after H(2)O(2) exposure. SIGNIFICANCE: We found that PME-1 is exported from the nucleus to the cytoplasm upon H(2)O(2) treatment and redistributes dem-p-PP2Ac in subcellular compartments. These findings offer new insight into the regulation of PME-1 localization and PP2A demethylation under oxidative stress. |
| ESTHER : Tang_2018_Life.Sci_213_166 |
| PubMedSearch : Tang_2018_Life.Sci_213_166 |
| PubMedID: 30340029 |
| Gene_locus related to this paper: human-PPME1 |
| Gene_locus related to this paper: human-PPME1 |
Tang S, Lu C, Mo L, Wang X, Liang Z, Qin F, Liu Y, Huang H, Huang Y, Cai H, Xiao D, Guo S, Ouyang Y, Sun B, Li X (2018)
Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1
Life Sciences
213 :166
Tang S, Lu C, Mo L, Wang X, Liang Z, Qin F, Liu Y, Huang H, Huang Y, Cai H, Xiao D, Guo S, Ouyang Y, Sun B, Li X (2018)
Life Sciences
213 :166