| Title : Discovery and Characterization of a PKS-NRPS Hybrid in Aspergillus terreus by Genome Mining - Tang_2020_J.Nat.Prod_83_473 |
| Author(s) : Tang S , Zhang W , Li Z , Li H , Geng C , Huang X , Lu X |
| Ref : Journal of Natural Products , 83 :473 , 2020 |
|
Abstract :
Fungal polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) hybrids have been characterized to produce polyketide-amino acid compounds with striking structural features and biological activities. In this study, a PKS-NRPS hybrid enzyme was found in Aspergillus terreus by genome mining. By activating the cluster-specific transcriptional regulator, this cryptic PKS-NRPS gene cluster was successfully activated and ten products (1-10) were identified as pyranterreones. Using functional genetics, bioinformatics, and isotope-labeling feeding analysis, the biosynthetic pathway was revealed. This is the second PKS-NRPS hybrid identified in A. terreus. |
| PubMedSearch : Tang_2020_J.Nat.Prod_83_473 |
| PubMedID: 32077283 |
| Gene_locus related to this paper: asptn-pytb , aspte-pyti |
| Gene_locus | asptn-pytb aspte-pyti |
Tang S, Zhang W, Li Z, Li H, Geng C, Huang X, Lu X (2020)
Discovery and Characterization of a PKS-NRPS Hybrid in Aspergillus terreus by Genome Mining
Journal of Natural Products
83 :473
Tang S, Zhang W, Li Z, Li H, Geng C, Huang X, Lu X (2020)
Journal of Natural Products
83 :473