Tanino_2007_Appl.Microbiol.Biotechnol_75_1319

Reference

Title : Development of yeast cells displaying Candida antarctica lipase B and their application to ester synthesis reaction - Tanino_2007_Appl.Microbiol.Biotechnol_75_1319
Author(s) : Tanino T , Ohno T , Aoki T , Fukuda H , Kondo A
Ref : Applied Microbiology & Biotechnology , 75 :1319 , 2007
Abstract :

We isolated the lipase B from Candida antarctica CBS 6678 (CALB CBS6678) and successfully constructed CALB-displaying yeast whole-cell biocatalysts using the Flo1p short (FS) anchor system. For the display of CALB on a yeast cell surface, the newly isolated CALB CBS6678 exhibited higher hydrolytic and ester synthesis activities than the well-known CALB, which is registered in GenBank (Z30645). A protease accessibility assay using papain as a protease showed that a large part of CALB, approximately 75%, was localized on an easily accessible part of the yeast cell surface. A comparison of the lipase hydrolytic activities of yeast whole cells displaying only mature CALB (CALB) and those displaying mature CALB with a Pro region (ProCALB) revealed that mature CALB is preferable for yeast cell surface display using the Flo1p anchor system. Lyophilized yeast whole cells displaying CALB were applied to an ester synthesis reaction at 60 degrees C using adipic acid and n-butanol as substrates. The amount of dibutyl adipate (DBA) produced increased with the reaction time until 144 h. This indicated that CALB displayed on the yeast cell surface retained activity under the reaction conditions.

PubMedSearch : Tanino_2007_Appl.Microbiol.Biotechnol_75_1319
PubMedID: 17406864

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Citations formats

Tanino T, Ohno T, Aoki T, Fukuda H, Kondo A (2007)
Development of yeast cells displaying Candida antarctica lipase B and their application to ester synthesis reaction
Applied Microbiology & Biotechnology 75 :1319

Tanino T, Ohno T, Aoki T, Fukuda H, Kondo A (2007)
Applied Microbiology & Biotechnology 75 :1319