Aoki T

References (17)

Title : Dipeptidyl Peptidase-4 Inhibitor Anagliptin Prevents Intracranial Aneurysm Growth by Suppressing Macrophage Infiltration and Activation - Ikedo_2017_J.Am.Heart.Assoc_6_
Author(s) : Ikedo T , Minami M , Kataoka H , Hayashi K , Nagata M , Fujikawa R , Higuchi S , Yasui M , Aoki T , Fukuda M , Yokode M , Miyamoto S
Ref : J Am Heart Assoc , 6 : , 2017
Abstract : BACKGROUND: Chronic inflammation plays a key role in the pathogenesis of intracranial aneurysms (IAs). DPP-4 (dipeptidyl peptidase-4) inhibitors have anti-inflammatory effects, including suppressing macrophage infiltration, in various inflammatory models. We examined whether a DPP-4 inhibitor, anagliptin, could suppress the growth of IAs in a rodent aneurysm model. METHODS AND RESULTS: IAs were surgically induced in 7-week-old male Sprague Dawley rats, followed by oral administration of 300 mg/kg anagliptin. We measured the morphologic parameters of aneurysms over time and their local inflammatory responses. To investigate the molecular mechanisms, we used lipopolysaccharide-treated RAW264.7 macrophages. In the anagliptin-treated group, aneurysms were significantly smaller 2 to 4 weeks after IA induction. Anagliptin inhibited the accumulation of macrophages in IAs, reduced the expression of MCP-1 (monocyte chemotactic protein 1), and suppressed the phosphorylation of p65. In lipopolysaccharide-stimulated RAW264.7 cells, anagliptin treatment significantly reduced the production of tumor necrosis factor alpha, MCP-1, and IL-6 (interleukin 6) independent of GLP-1 (glucagon-like peptide 1), the key mediator in the antidiabetic effects of DPP-4 inhibitors. Notably, anagliptin activated ERK5 (extracellular signal-regulated kinase 5), which mediates the anti-inflammatory effects of statins, in RAW264.7 macrophages. Preadministration with an ERK5 inhibitor blocked the inhibitory effect of anagliptin on MCP-1 and IL-6 expression. Accordingly, the ERK5 inhibitor also counteracted the suppression of p65 phosphorylation in vitro. CONCLUSIONS: A DPP-4 inhibitor, anagliptin, prevents the growth of IAs via its anti-inflammatory effects on macrophages.
ESTHER : Ikedo_2017_J.Am.Heart.Assoc_6_
PubMedSearch : Ikedo_2017_J.Am.Heart.Assoc_6_
PubMedID: 28630262

Title : A new metabolism-related index correlates with the degree of liver fibrosis in hepatitis C virus-positive patients - Enomoto_2015_Gastroenterol.Res.Pract_2015_926169
Author(s) : Enomoto H , Aizawa N , Nakamura H , Takata R , Sakai Y , Iwata Y , Tanaka H , Ikeda N , Aoki T , Hasegawa K , Yoh K , Hashimoto K , Ishii A , Takashima T , Saito M , Imanishi H , Iijima H , Nishiguchi S
Ref : Gastroenterol Res Pract , 2015 :926169 , 2015
Abstract : Background. Only a few biomarkers based on metabolic parameters for evaluating liver fibrosis have been reported. The aim of this study was to investigate the relevance of an index obtained from three metabolic variables (glycated albumin: GA, glycated hemoglobin: HbA1c, and branched-chain amino acids to tyrosine ratio: BTR) to the degree of liver fibrosis in hepatitis C virus virus- (HCV-) positive patients. Methods. A total of 394 HCV-positive patients were assessed based on the values of a new index (GA/HbA1c/BTR). The index findings were used to investigate the relationship with the degree of liver fibrosis. Results. The new index showed an association with the stage of fibrosis (METAVIR scores: F0-1: 0.42 +/- 0.10, F2: 0.48 +/- 0.15, F3: 0.56 +/- 0.22, and F4: 0.71 +/- 0.30). The index was negatively correlated with three variables of liver function: the prothrombin time percentage (P < 0.0001), albumin level (P < 0.0001), and cholinesterase level (P < 0.0001). The new index showed a higher correlation related to liver function than FIB-4 and the APRI did. In addition, the index showed a higher AUROC value than that of FIB-4 and the APRI for prediction of liver cirrhosis. Conclusion. The new metabolism-related index, GA/HbA1c/BTR value, is shown to relate to the degree of liver fibrosis in HCV-positive patients.
ESTHER : Enomoto_2015_Gastroenterol.Res.Pract_2015_926169
PubMedSearch : Enomoto_2015_Gastroenterol.Res.Pract_2015_926169
PubMedID: 25861264

Title : Lung cancer associated with seronegative myasthenia gravis - Niimi_2015_Intern.Med_54_1381
Author(s) : Niimi K , Nagata E , Murata N , Sato M , Tanaka J , Horio Y , Takiguchi H , Tomomatsu H , Tomomatsu K , Hayama N , Oguma T , Aoki T , Urano T , Abe T , Inomoto C , Takizawa S , Asano K
Ref : Intern Med , 54 :1381 , 2015
Abstract : A 64-year-old man presented with diplopia, muscle weakness, a pulmonary nodule and mediastinal widening on a chest radiograph. He was diagnosed with clinical stage IIIA (T2aN2M0) lung cancer. His neurological symptoms worsened following the initiation of thoracic radiation therapy (60 Gy) and chemotherapy. A diagnosis of myasthenia gravis (MG) was confirmed with a repetitive nerve stimulation test that showed a waning pattern, and a positive edrophonium test, although neither anti-acetylcholine receptor antibodies nor anti-muscle-specific tyrosine kinase antibodies were detected. The ptosis and limb muscle weakness improved with prednisolone and acetylcholinesterase inhibitor treatment, and a partial response of the lung cancer to chemoradiotherapy was obtained. However, the ptosis and limb muscle weakness worsened again following a recurrence of the lung cancer. The herein described case, in which lung cancer and MG occurred and recurred simultaneously, suggests that MG can develop as a paraneoplastic syndrome of lung cancer.
ESTHER : Niimi_2015_Intern.Med_54_1381
PubMedSearch : Niimi_2015_Intern.Med_54_1381
PubMedID: 26027991

Title : An Increased Ratio of Glycated Albumin to HbA1c Is Associated with the Degree of Liver Fibrosis in Hepatitis B Virus-Positive Patients - Enomoto_2014_Gastroenterol.Res.Pract_2014_351396
Author(s) : Enomoto H , Aizawa N , Nakamura H , Sakai Y , Iwata Y , Tanaka H , Ikeda N , Aoki T , Yuri Y , Yoh K , Hashimoto K , Ishii A , Takashima T , Iwata K , Saito M , Imanishi H , Iijima H , Nishiguchi S
Ref : Gastroenterol Res Pract , 2014 :351396 , 2014
Abstract : Background. In hepatitis B virus- (HBV-) positive patients, the relationship between the metabolic variables and histological degree of liver fibrosis has been poorly investigated. Methods. A total of 176 HBV-positive patients were assessed in whom the ratios of glycated albumin-to-glycated hemoglobin (GA/HbA1c) were calculated in order to investigate the relationship with the degree of liver fibrosis. Results. The GA/HbA1c ratio increased in association with the severity of fibrosis (METAVIR scores: F0-1: 2.61 +/- 0.24, F2: 2.65 +/- 0.24, F3: 2.74 +/- 0.38, and F4: 2.91 +/- 0.63). The GA/HbA1c ratios were inversely correlated with four variables of liver function: the prothrombin time (PT) percentage (P < 0.0001), platelet count (P < 0.0001), albumin value (P < 0.0001), and cholinesterase value (P < 0.0001). The GA/HbA1c ratio was positively correlated with two well-known markers of liver fibrosis, FIB-4 (P < 0.0001) and the AST-to-platelet ratio index (APRI) (P < 0.0001). Furthermore, the GA/HbA1c showed better correlations with two variables of liver function (PT percentage and cholinesterase value) than did FIB-4 and with all four variables than did the APRI. Conclusion. The GA/HbA1c ratio is associated with the degree of liver fibrosis in HBV-positive patients.
ESTHER : Enomoto_2014_Gastroenterol.Res.Pract_2014_351396
PubMedSearch : Enomoto_2014_Gastroenterol.Res.Pract_2014_351396
PubMedID: 24693282

Title : Discovery of 1-oxa-4,9-diazaspiro[5.5]undecane-based trisubstituted urea derivatives as highly potent soluble epoxide hydrolase inhibitors and orally active drug candidates for treating of chronic kidney diseases - Kato_2014_Bioorg.Med.Chem.Lett_24_565
Author(s) : Kato Y , Fuchi N , Nishimura Y , Watanabe A , Yagi M , Nakadera Y , Higashi E , Yamada M , Aoki T , Kigoshi H
Ref : Bioorganic & Medicinal Chemistry Lett , 24 :565 , 2014
Abstract : We identified 1-oxa-4,9-diazaspiro[5.5]undecane-based trisubstituted ureas as highly potent soluble epoxide hydrolase (sEH) inhibitors and orally active agents for treating chronic kidney diseases. Compound 19 exhibited excellent sEH inhibitory activity and bioavailability. When administered orally at 30 mg/kg, 19 lowered serum creatinine in a rat model of anti-glomerular basement membrane glomerulonephritis but 2,8-diazaspiro[4.5]decane-based trisubstituted ureas did not. These results suggest that 19 is an orally active drug candidate for treating chronic kidney diseases.
ESTHER : Kato_2014_Bioorg.Med.Chem.Lett_24_565
PubMedSearch : Kato_2014_Bioorg.Med.Chem.Lett_24_565
PubMedID: 24373724

Title : Comparative genomic characterization of three Streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses - Nho_2013_PLoS.One_8_e80395
Author(s) : Nho SW , Hikima J , Park SB , Jang HB , Cha IS , Yasuike M , Nakamura Y , Fujiwara A , Sano M , Kanai K , Kondo H , Hirono I , Takeyama H , Aoki T , Jung TS
Ref : PLoS ONE , 8 :e80395 , 2013
Abstract : Streptococcus parauberis, which is the main causative agent of streptococcosis among olive flounder (Paralichthys olivaceus) in northeast Asia, can be distinctly divided into two groups (type I and type II) by an agglutination test. Here, the whole genome sequences of two Japanese strains (KRS-02083 and KRS-02109) were determined and compared with the previously determined genome of a Korean strain (KCTC 11537). The genomes of S. parauberis are intermediate in size and have lower GC contents than those of other streptococci. We annotated 2,236 and 2,048 genes in KRS-02083 and KRS-02109, respectively. Our results revealed that the three S. parauberis strains contain different genomic insertions and deletions. In particular, the genomes of Korean and Japanese strains encode different factors for sugar utilization; the former encodes the phosphotransferase system (PTS) for sorbose, whereas the latter encodes proteins for lactose hydrolysis, respectively. And the KRS-02109 strain, specifically, was the type II strain found to be able to resist phage infection through the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system and which might contribute valuably to serologically distribution. Thus, our genome-wide association study shows that polymorphisms can affect pathogen responses, providing insight into biological/biochemical pathways and phylogenetic diversity.
ESTHER : Nho_2013_PLoS.One_8_e80395
PubMedSearch : Nho_2013_PLoS.One_8_e80395
PubMedID: 24260382
Gene_locus related to this paper: 9stre-f1yze3

Title : Whole-Genome Sequence of Fish-Pathogenic Mycobacterium sp. Strain 012931, Isolated from Yellowtail (Seriola quinqueradiata) - Kurokawa_2013_Genome.Announc_1_e00534
Author(s) : Kurokawa S , Kabayama J , Nho SW , Hwang SD , Hikima J , Jung TS , Kondo H , Hirono I , Takeyama H , Aoki T
Ref : Genome Announc , 1 : , 2013
Abstract : The genus Mycobacterium comprises a large number of well-characterized species, several of which are human and animal pathogens. Here, we report the whole-genome sequence of Mycobacterium sp. strain 012931, a fish pathogen responsible for huge losses in aquaculture farms in Japan. The strain was isolated from a marine fish, yellowtail (Seriola quinqueradiata).
ESTHER : Kurokawa_2013_Genome.Announc_1_e00534
PubMedSearch : Kurokawa_2013_Genome.Announc_1_e00534
PubMedID: 23929466
Gene_locus related to this paper: mycmm-b2hjb4 , mycmm-b2ht49 , mycmm-dhma , mycua-a0pqm2

Title : Discovery of 2,8-diazaspiro[4.5]decane-based trisubstituted urea derivatives as highly potent soluble epoxide hydrolase inhibitors and orally active drug candidates for treating hypertension - Kato_2013_Bioorg.Med.Chem.Lett_23_5975
Author(s) : Kato Y , Fuchi N , Saburi H , Nishimura Y , Watanabe A , Yagi M , Nakadera Y , Higashi E , Yamada M , Aoki T
Ref : Bioorganic & Medicinal Chemistry Lett , 23 :5975 , 2013
Abstract : We identified 2,8-diazaspiro[4.5]decane-based trisubstituted urea derivatives as highly potent soluble epoxide hydrolase (sEH) inhibitors and orally active agents for treating hypertension. Docking studies using human and murine sEH X-ray crystal structures revealed steric hindrance around the side chain of Phe406 of murine sEH. The trifluoromethyl moiety (11) was replaced with a trifluoromethoxy moiety (12) to prevent steric clash, and improved murine sEH inhibitory activity was observed. The oral administration of 12, 20, and 37 at a dose of 30mg/kg reduced blood pressure in spontaneously hypertensive rat, but had little effect on blood pressure in normotensive rat.
ESTHER : Kato_2013_Bioorg.Med.Chem.Lett_23_5975
PubMedSearch : Kato_2013_Bioorg.Med.Chem.Lett_23_5975
PubMedID: 24035338

Title : Nephrotic syndrome caused by immune-mediated acquired LCAT deficiency - Takahashi_2013_J.Am.Soc.Nephrol_24_1305
Author(s) : Takahashi S , Hiromura K , Tsukida M , Ohishi Y , Hamatani H , Sakurai N , Sakairi T , Ikeuchi H , Kaneko Y , Maeshima A , Kuroiwa T , Yokoo H , Aoki T , Nagata M , Nojima Y
Ref : J Am Soc Nephrol , 24 :1305 , 2013
Abstract : Lecithin-cholesterol acyltransferase (LCAT) is an enzyme involved in maintaining cholesterol homeostasis. In familial LCAT deficiency (FLD), abnormal lipid deposition causes renal injury and nephrotic syndrome, frequently progressing to ESRD. Here, we describe a 63-year-old Japanese woman with no family history of renal disease who presented with nephrotic syndrome. The laboratory data revealed an extremely low level of serum HDL and undetectable serum LCAT activity. Renal biopsy showed glomerular lipid deposition with prominent accumulation of foam cells, similar to the histologic findings of FLD. In addition, she had subepithelial electron-dense deposits compatible with membranous nephropathy, which are not typical of FLD. A mixing test and coimmunoprecipitation study demonstrated the presence of an inhibitory anti-LCAT antibody in the patient's serum. Immunohistochemistry and immunofluorescence detected LCAT along parts of the glomerular capillary walls, suggesting that LCAT was an antigen responsible for the membranous nephropathy. Treatment with steroids resulted in complete remission of the nephrotic syndrome, normalization of serum LCAT activity and HDL level, and disappearance of foam cell accumulation in renal tissue. In summary, inhibitory anti-LCAT antibody can lead to glomerular lesions similar to those observed in FLD.
ESTHER : Takahashi_2013_J.Am.Soc.Nephrol_24_1305
PubMedSearch : Takahashi_2013_J.Am.Soc.Nephrol_24_1305
PubMedID: 23620397
Gene_locus related to this paper: human-LCAT

Title : Complete genome sequence and immunoproteomic analyses of the bacterial fish pathogen Streptococcus parauberis - Nho_2011_J.Bacteriol_193_3356
Author(s) : Nho SW , Hikima J , Cha IS , Park SB , Jang HB , del Castillo CS , Kondo H , Hirono I , Aoki T , Jung TS
Ref : Journal of Bacteriology , 193 :3356 , 2011
Abstract : Although Streptococcus parauberis is known as a bacterial pathogen associated with bovine udder mastitis, it has recently become one of the major causative agents of olive flounder (Paralichthys olivaceus) streptococcosis in northeast Asia, causing massive mortality resulting in severe economic losses. S. parauberis contains two serotypes, and it is likely that capsular polysaccharide antigens serve to differentiate the serotypes. In the present study, the complete genome sequence of S. parauberis (serotype I) was determined using the GS-FLX system to investigate its phylogeny, virulence factors, and antigenic proteins. S. parauberis possesses a single chromosome of 2,143,887 bp containing 1,868 predicted coding sequences (CDSs), with an average GC content of 35.6%. Whole-genome dot plot analysis and phylogenetic analysis of a 60-kDa chaperonin-encoding gene and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-encoding gene showed that the strain was evolutionarily closely related to Streptococcus uberis. S. parauberis antigenic proteins were analyzed using an immunoproteomic technique. Twenty-one antigenic protein spots were identified in S. parauberis, by reaction with an antiserum obtained from S. parauberis-challenged olive flounder. This work provides the foundation needed to understand more clearly the relationship between pathogen and host and develops new approaches toward prophylactic and therapeutic strategies to deal with streptococcosis in fish. The work also provides a better understanding of the physiology and evolution of a significant representative of the Streptococcaceae.
ESTHER : Nho_2011_J.Bacteriol_193_3356
PubMedSearch : Nho_2011_J.Bacteriol_193_3356
PubMedID: 21531805
Gene_locus related to this paper: 9stre-f1yze3

Title : Improvement of a Candida antarctica lipase B-displaying yeast whole-cell biocatalyst and its application to the polyester synthesis reaction - Tanino_2009_Appl.Microbiol.Biotechnol_82_59
Author(s) : Tanino T , Aoki T , Chung WY , Watanabe Y , Ogino C , Fukuda H , Kondo A
Ref : Applied Microbiology & Biotechnology , 82 :59 , 2009
Abstract : A Candida antarctica lipase B (CALB)-displaying yeast whole-cell biocatalyst was constructed with the integration of the CALB cell-surface display expression cassette in the yeast genome and cell fusion by mating. Lipase hydrolytic activity of the yeast whole-cell biocatalyst subsequently increased, in both a- and alpha-type yeast cells, with the number of copies of the CALB cell-surface display expression cassette introduced, and reached 43.6 and 32.2 U/g-dry cell at 168 h cultivation, respectively. The lipase hydrolytic activity of whole cells in diploid yeast cells containing eight copies of the CALB cell-surface expression cassette reached 117 U/g-dry cell, and this value is approximately ninefold higher than that of the previously reported haploid CALB cell-surface displaying yeast using a multi-copy plasmid (Tanino et al. Appl. Microbial Biotechnol 75:1319-1325, 2007). This improved novel CALB-displaying yeast whole-cell biocatalyst could repeatedly catalyze the polyester, polybutylene adipate, synthesis reaction, using adipic acid and 1, 4-butandiol as the monomer molecules, four times in succession. This is the first report of the polymer synthesis using enzyme displaying yeast as the catalyst. The ratios of cyclic compounds in the polybutylene adipates synthesized with the CALB-displaying yeast whole-cells were lower than that in the polybutylene adipate synthesized with conventional metal catalysis. From these results, it appears that the use of CALB-displaying yeast cells could be useful for the polyester synthesis reaction, with reduced by-product production.
ESTHER : Tanino_2009_Appl.Microbiol.Biotechnol_82_59
PubMedSearch : Tanino_2009_Appl.Microbiol.Biotechnol_82_59
PubMedID: 18985336

Title : Complete DNA sequence and analysis of the transferable multiple-drug resistance plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida isolates collected in Japan and the United States - Kim_2008_Antimicrob.Agents.Chemother_52_606
Author(s) : Kim MJ , Hirono I , Kurokawa K , Maki T , Hawke J , Kondo H , Santos MD , Aoki T
Ref : Antimicrobial Agents & Chemotherapy , 52 :606 , 2008
Abstract : Photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. Two transferable multiple-drug resistance (R) plasmids, pP99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pP91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from P. damselae subsp. piscicida strains from Japan (P99-018) and the United States (P91278), respectively, were completely sequenced and analyzed, along with the multiple-drug resistance regions of three other R plasmids also from P. damselae subsp. piscicida strains from Japan. The sequence structures of pP99-018 (150,057 bp) and pP91278 (131,520 bp) were highly conserved, with differences due to variation in the drug resistance and conjugative transfer regions. These plasmids, shown to be closely related to the IncJ element R391 (a conjugative, self-transmitting, integrating element, or constin), were divided into the conjugative transfer, replication, partition, and multiple-drug resistance regions. Each of the five multiple-drug resistance regions sequenced exhibited unique drug resistance marker composition and arrangement.
ESTHER : Kim_2008_Antimicrob.Agents.Chemother_52_606
PubMedSearch : Kim_2008_Antimicrob.Agents.Chemother_52_606
PubMedID: 18070959

Title : Multilocus genotyping and molecular phylogenetics resolve a novel head blight pathogen within the Fusarium graminearum species complex from Ethiopia - O'Donnell_2008_Fungal.Genet.Biol_45_1514
Author(s) : O'Donnell K , Ward TJ , Aberra D , Kistler HC , Aoki T , Orwig N , Kimura M , Bjornstad S , Klemsdal SS
Ref : Fungal Genet Biol , 45 :1514 , 2008
Abstract : A survey of Fusarium head blight (FHB)-contaminated wheat in Ethiopia recovered 31 isolates resembling members of the Fusarium graminearum species complex. Results of a multilocus genotyping (MLGT) assay for FHB species and trichothecene chemotype determination suggested that 22 of these isolates might represent a new species within the Fg complex. Phylogenetic analyses of multilocus DNA sequence data resolved the 22 Ethiopian isolates as a novel, phylogenetically distinct species. The new species also appears to be novel in that MLGT probe data and sequence analysis of both ends of the TRI-cluster identified 15ADON and NIV recombination blocks, documenting inter-chemotype recombination involving the chemotype-determining genes near the ends of the TRI-cluster. Results of pathogenicity experiments and analyses of trichothecene mycotoxins demonstrated that this novel Fg complex species could induce FHB on wheat and elaborate 15ADON in planta. Herein the FHB pathogen from Ethiopia is formally described as a novel species.
ESTHER : O'Donnell_2008_Fungal.Genet.Biol_45_1514
PubMedSearch : O'Donnell_2008_Fungal.Genet.Biol_45_1514
PubMedID: 18824240
Gene_locus related to this paper: gibze-TRI8

Title : Cy3-3-acylcholine: a fluorescent analogue of acetylcholine for single molecule detection - Fujimoto_2008_Bioorg.Med.Chem.Lett_18_1106
Author(s) : Fujimoto K , Yoshimura Y , Ihara M , Matsuda K , Takeuchi Y , Aoki T , Ide T
Ref : Bioorganic & Medicinal Chemistry Lett , 18 :1106 , 2008
Abstract : We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.
ESTHER : Fujimoto_2008_Bioorg.Med.Chem.Lett_18_1106
PubMedSearch : Fujimoto_2008_Bioorg.Med.Chem.Lett_18_1106
PubMedID: 18155907

Title : Development of yeast cells displaying Candida antarctica lipase B and their application to ester synthesis reaction - Tanino_2007_Appl.Microbiol.Biotechnol_75_1319
Author(s) : Tanino T , Ohno T , Aoki T , Fukuda H , Kondo A
Ref : Applied Microbiology & Biotechnology , 75 :1319 , 2007
Abstract : We isolated the lipase B from Candida antarctica CBS 6678 (CALB CBS6678) and successfully constructed CALB-displaying yeast whole-cell biocatalysts using the Flo1p short (FS) anchor system. For the display of CALB on a yeast cell surface, the newly isolated CALB CBS6678 exhibited higher hydrolytic and ester synthesis activities than the well-known CALB, which is registered in GenBank (Z30645). A protease accessibility assay using papain as a protease showed that a large part of CALB, approximately 75%, was localized on an easily accessible part of the yeast cell surface. A comparison of the lipase hydrolytic activities of yeast whole cells displaying only mature CALB (CALB) and those displaying mature CALB with a Pro region (ProCALB) revealed that mature CALB is preferable for yeast cell surface display using the Flo1p anchor system. Lyophilized yeast whole cells displaying CALB were applied to an ester synthesis reaction at 60 degrees C using adipic acid and n-butanol as substrates. The amount of dibutyl adipate (DBA) produced increased with the reaction time until 144 h. This indicated that CALB displayed on the yeast cell surface retained activity under the reaction conditions.
ESTHER : Tanino_2007_Appl.Microbiol.Biotechnol_75_1319
PubMedSearch : Tanino_2007_Appl.Microbiol.Biotechnol_75_1319
PubMedID: 17406864

Title : Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis - Akashi_2005_Plant.Physiol_137_882
Author(s) : Akashi T , Aoki T , Ayabe S
Ref : Plant Physiol , 137 :882 , 2005
Abstract : Isoflavonoids are ecophysiologically active secondary metabolites of the Leguminosae and known for health-promoting phytoestrogenic functions. Isoflavones are synthesized by 1,2-elimination of water from 2-hydroxyisoflavanones, the first intermediate with the isoflavonoid skeleton, but details of this dehydration have been unclear. We screened the extracts of repeatedly fractionated Escherichia coli expressing a Glycyrrhiza echinata cDNA library for the activity to convert a radiolabeled precursor into formononetin (7-hydroxy-4'-methoxyisoflavone), and a clone of 2-hydroxyisoflavanone dehydratase (HID) was isolated. Another HID cDNA was cloned from soybean (Glycine max), based on the sequence information in its expressed sequence tag library. Kinetic studies revealed that G. echinata HID is specific to 2,7-dihydroxy-4'-methoxyisoflavanone, while soybean HID has broader specificity to both 4'-hydroxylated and 4'-methoxylated 2-hydroxyisoflavanones, reflecting the structures of isoflavones contained in each plant species. Strikingly, HID proteins were members of a large carboxylesterase family, of which plant proteins form a monophyletic group and some are assigned defensive functions with no intrinsic catalytic activities identified. Site-directed mutagenesis with soybean HID protein suggested that the characteristic oxyanion hole and catalytic triad are essential for the dehydratase as well as the faint esterase activities. The findings, to our knowledge, represent a new example of recruitment of enzymes of primary metabolism during the molecular evolution of plant secondary metabolism.
ESTHER : Akashi_2005_Plant.Physiol_137_882
PubMedSearch : Akashi_2005_Plant.Physiol_137_882
PubMedID: 15734910
Gene_locus related to this paper: glyec-q5nuf4 , soybn-q5nuf3

Title : Failure to produce axon reflex-sweating in the volar skin of Japanese monkey (Macaca fuscata) and crab-eating monkey (Macaca irus) - Aoki_1984_Comp.Biochem.Physiol.C_79_325
Author(s) : Aoki T , Narita T , Sato H , Izumi H
Ref : Comparative Biochemistry & Physiology C , 79 :325 , 1984
Abstract : The functional properties of the sweat glands and their innervation in the volar skin of three Japanese monkeys and two crab-eating monkeys were investigated. The sweat glands responded to both cholinomimetic and adrenomimetic agents, the former being highly predominant in the sudorific effect. Spontaneous emotional sweating was strongly or completely inhibited by atropine at 10(-8)-10(-7) g/ml, but not by dihydroergotamine at 10(-5)-10(-4) g/ml. Axon reflex sweating could not be produced by nicotine at 10(-5)-10(-4) g/ml in all of primates tested. The nerve fibers surrounding the sweat glands were histochemically confirmed to contain both acetyl- and butyrylcholinesterase.
ESTHER : Aoki_1984_Comp.Biochem.Physiol.C_79_325
PubMedSearch : Aoki_1984_Comp.Biochem.Physiol.C_79_325
PubMedID: 6151462