Torrecilla_2007_J.Cell.Biol_177_127

Reference

Title : Phosphorylation and regulation of a G protein-coupled receptor by protein kinase CK2 - Torrecilla_2007_J.Cell.Biol_177_127
Author(s) : Torrecilla I , Spragg EJ , Poulin B , McWilliams PJ , Mistry SC , Blaukat A , Tobin AB
Ref : Journal of Cell Biology , 177 :127 , 2007
Abstract :

We demonstrate a role for protein kinase casein kinase 2 (CK2) in the phosphorylation and regulation of the M3-muscarinic receptor in transfected cells and cerebellar granule neurons. On agonist occupation, specific subsets of receptor phosphoacceptor sites (which include the SASSDEED motif in the third intracellular loop) are phosphorylated by CK2. Receptor phosphorylation mediated by CK2 specifically regulates receptor coupling to the Jun-kinase pathway. Importantly, other phosphorylation-dependent receptor processes are regulated by kinases distinct from CK2. We conclude that G protein-coupled receptors (GPCRs) can be phosphorylated in an agonist-dependent fashion by protein kinases from a diverse range of kinase families, not just the GPCR kinases, and that receptor phosphorylation by a defined kinase determines a specific signalling outcome. Furthermore, we demonstrate that the M3-muscarinic receptor can be differentially phosphorylated in different cell types, indicating that phosphorylation is a flexible regulatory process where the sites that are phosphorylated, and hence the signalling outcome, are dependent on the cell type in which the receptor is expressed.

PubMedSearch : Torrecilla_2007_J.Cell.Biol_177_127
PubMedID: 17403928

Related information

Citations formats

Torrecilla I, Spragg EJ, Poulin B, McWilliams PJ, Mistry SC, Blaukat A, Tobin AB (2007)
Phosphorylation and regulation of a G protein-coupled receptor by protein kinase CK2
Journal of Cell Biology 177 :127

Torrecilla I, Spragg EJ, Poulin B, McWilliams PJ, Mistry SC, Blaukat A, Tobin AB (2007)
Journal of Cell Biology 177 :127