Traeger_2013_PLoS.Genet_9_E1003820

Reference

Title : The genome and development-dependent transcriptomes of Pyronema confluens: a window into fungal evolution - Traeger_2013_PLoS.Genet_9_e1003820
Author(s) : Traeger S , Altegoer F , Freitag M , Gabaldon T , Kempken F , Kumar A , Marcet-Houben M , Poggeler S , Stajich JE , Nowrousian M
Ref : PLoS Genet , 9 :e1003820 , 2013
Abstract :

Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding of fungal evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage of filamentous ascomycetes, there is so far only one genome available, namely that of the black truffle, Tuber melanosporum, a mycorrhizal species with unusual subterranean fruiting bodies. To help close the sequence gap among basal filamentous ascomycetes, and to allow conclusions about the evolution of fungal development, we sequenced the genome and assayed transcriptomes during development of Pyronema confluens, a saprobic Pezizomycete with a typical apothecium as fruiting body. With a size of 50 Mb and ~13,400 protein-coding genes, the genome is more characteristic of higher filamentous ascomycetes than the large, repeat-rich truffle genome; however, some typical features are different in the P. confluens lineage, e.g. the genomic environment of the mating type genes that is conserved in higher filamentous ascomycetes, but only partly conserved in P. confluens. On the other hand, P. confluens has a full complement of fungal photoreceptors, and expression studies indicate that light perception might be similar to distantly related ascomycetes and, thus, represent a basic feature of filamentous ascomycetes. Analysis of spliced RNA-seq sequence reads allowed the detection of natural antisense transcripts for 281 genes. The P. confluens genome contains an unusually high number of predicted orphan genes, many of which are upregulated during sexual development, consistent with the idea of rapid evolution of sex-associated genes. Comparative transcriptomics identified the transcription factor gene pro44 that is upregulated during development in P. confluens and the Sordariomycete Sordaria macrospora. The P. confluens pro44 gene (PCON_06721) was used to complement the S. macrospora pro44 deletion mutant, showing functional conservation of this developmental regulator.

PubMedSearch : Traeger_2013_PLoS.Genet_9_e1003820
PubMedID: 24068976
Gene_locus related to this paper: pyrom-u4ltg1 , pyrom-u4lhe1 , pyrom-u4kwa3 , pyrom-u4lfu9 , pyrom-u4l0a5 , pyrom-u4l0n5 , pyrom-u4lhv4 , pyrom-u4l8w9 , pyrom-u4lvb8 , pyrom-u4l9c9 , pyrom-u4lwe6 , pyrom-u4l3n9

Related information

Gene_locus pyrom-u4ltg1    pyrom-u4lhe1    pyrom-u4kwa3    pyrom-u4lfu9    pyrom-u4l0a5    pyrom-u4l0n5    pyrom-u4lhv4    pyrom-u4l8w9    pyrom-u4lvb8    pyrom-u4l9c9    pyrom-u4lwe6    pyrom-u4l3n9
Gene_locus_frgt pyrom-u4ltg1    pyrom-u4lhe1    pyrom-u4kwa3    pyrom-u4lfu9    pyrom-u4l0a5    pyrom-u4l0n5    pyrom-u4lhv4    pyrom-u4l8w9    pyrom-u4lvb8    pyrom-u4l9c9    pyrom-u4lwe6    pyrom-u4l3n9    pyrom-u4l645    pyrom-u4l2n5

Citations formats

Traeger S, Altegoer F, Freitag M, Gabaldon T, Kempken F, Kumar A, Marcet-Houben M, Poggeler S, Stajich JE, Nowrousian M (2013)
The genome and development-dependent transcriptomes of Pyronema confluens: a window into fungal evolution
PLoS Genet 9 :e1003820

Traeger S, Altegoer F, Freitag M, Gabaldon T, Kempken F, Kumar A, Marcet-Houben M, Poggeler S, Stajich JE, Nowrousian M (2013)
PLoS Genet 9 :e1003820