| Title : Neuroprotective activity of isoquinoline alkaloids from of Chilean Amaryllidaceae plants against oxidative stress-induced cytotoxicity on human neuroblastoma SH-SY5Y cells and mouse hippocampal slice culture - Trujillo-Chacon_2019_Food.Chem.Toxicol_132_110665 |
| Author(s) : Trujillo-Chacon LM , Alarcon-Enos JE , Cespedes-Acuna CL , Bustamante L , Baeza M , Lopez MG , Fernandez-Mendivil C , Cabezas F , Pastene-Navarrete ER |
| Ref : Food & Chemical Toxicology , 132 :110665 , 2019 |
| Abstract : In this study we evaluate the chemical composition and neuroprotective effects of alkaloid fractions of the Amaryllidaceae species Rhodophiala pratensis, Rhodolirium speciosum, Phycella australis and Phaedranassa lehmannii. Gas chromatography-mass spectrometry (GC/MS) enable the identification of 41 known alkaloids. Rhodolirium speciosum and Rhodophiala pratensis were the most active extracts against acetylcholinesterase (AChE), with IC50 values of 35.22 and 38.13mug/mL, respectively. The protective effect of these extracts on human neuroblastoma cells (SH-SY5Y) subjected to mitochondrial oxidative stress with rotenone/oligomycin A (R/O) and toxicity promoted by okadaic acid (OA) was evaluated. Only Phycella australis and Rhodophiala pratensis at 0.75 and 1.5mug/mL, tend to reverse the cell death induced by R/O by around 12%. In OA assay, alkaloid fractions of Phycella Australis and Phaedranassa lehmannii displayed a concentration-dependent (0.375-3.0mug/mL) effect with a maximum neuroprotective response of 78% and 84%, respectively. Afterwards, neuroprotective effects of Phycella australis (3 and 6mug/mL) in mouse hippocampal slices stressed with oxygen glucose deprivation/reoxygenation (OGD/R), shown a protection greater than 14%. Finally, Phycella Australis (6mug/mL) reverted the cell viability from 65% to 90% in slices treated with OA, representing a protection of 25% attributable to the alkaloids of this species. |
| ESTHER : Trujillo-Chacon_2019_Food.Chem.Toxicol_132_110665 |
| PubMedSearch : Trujillo-Chacon_2019_Food.Chem.Toxicol_132_110665 |
| PubMedID: 31283947 |
Trujillo-Chacon LM, Alarcon-Enos JE, Cespedes-Acuna CL, Bustamante L, Baeza M, Lopez MG, Fernandez-Mendivil C, Cabezas F, Pastene-Navarrete ER (2019)
Neuroprotective activity of isoquinoline alkaloids from of Chilean Amaryllidaceae plants against oxidative stress-induced cytotoxicity on human neuroblastoma SH-SY5Y cells and mouse hippocampal slice culture
Food & Chemical Toxicology
132 :110665
Trujillo-Chacon LM, Alarcon-Enos JE, Cespedes-Acuna CL, Bustamante L, Baeza M, Lopez MG, Fernandez-Mendivil C, Cabezas F, Pastene-Navarrete ER (2019)
Food & Chemical Toxicology
132 :110665