Tsuchiya_1999_Proc.Natl.Acad.Sci.U.S.A_96_15362

Reference

Title : Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate - Tsuchiya_1999_Proc.Natl.Acad.Sci.U.S.A_96_15362
Author(s) : Tsuchiya T , Ohta H , Okawa K , Iwamatsu A , Shimada H , Masuda T , Takamiya K
Ref : Proc Natl Acad Sci U S A , 96 :15362 , 1999
Abstract :

Chlorophyllase (Chlase) is the first enzyme involved in chlorophyll (Chl) degradation and catalyzes the hydrolysis of ester bond to yield chlorophyllide and phytol. In the present study, we isolated the Chlase cDNA. We synthesized degenerate oligo DNA probes based on the internal amino acid sequences of purified Chlase from Chenopodium album, screened the C. album cDNA library, and cloned a cDNA (CaCLH, C. album chlorophyll-chlorophyllido hydrolase). The deduced amino acid sequence (347 aa residues) had a lipase motif overlapping with an ATP/GTP-binding motif (P-loop). CaCLH possibly was localized in the extraplastidic part of the cell, because a putative signal sequence for endoplasmic reticulum is at the N terminus. The amino acid sequence shared 37% identity with a function-unknown gene whose mRNA is inducible by coronatine and methyl jasmonate (MeJA) in Arabidopsis thaliana (AtCLH1). We expressed the gene products of AtCLH1 and of CaCLH in Escherichia coli, and they similarly exhibited Chlase activity. Moreover, we isolated another full-length cDNA based on an Arabidopsis genomic fragment and expressed it in E. coli, demonstrating the presence of the second Arabidopsis CLH gene (AtCLH2). No typical feature of signal sequence was identified in AtCLH1, whereas AtCLH2 had a typical signal sequence for chloroplast. AtCLH1 mRNA was induced rapidly by a treatment of MeJA, which is known to promote senescence and Chl degradation in plants, and a high mRNA level was maintained up to 9 h. AtCLH2, however, did not respond to MeJA.

PubMedSearch : Tsuchiya_1999_Proc.Natl.Acad.Sci.U.S.A_96_15362
PubMedID: 10611389
Gene_locus related to this paper: arath-clh1 , arath-clh2 , cheal-CACLH

Related information

Gene_locus arath-clh1    arath-clh2    cheal-CACLH
Family arath-clh1    arath-clh2    cheal-CACLH    Chlorophyllase_Plant

Citations formats

Tsuchiya T, Ohta H, Okawa K, Iwamatsu A, Shimada H, Masuda T, Takamiya K (1999)
Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate
Proc Natl Acad Sci U S A 96 :15362

Tsuchiya T, Ohta H, Okawa K, Iwamatsu A, Shimada H, Masuda T, Takamiya K (1999)
Proc Natl Acad Sci U S A 96 :15362