Uraji_2014_PLoS.One_9_e104584

Reference

Title : Application of two newly identified and characterized feruloyl esterases from Streptomyces sp. in the enzymatic production of ferulic acid from agricultural biomass - Uraji_2014_PLoS.One_9_e104584
Author(s) : Uraji M , Arima J , Inoue Y , Harazono K , Hatanaka T
Ref : PLoS ONE , 9 :e104584 , 2014
Abstract :

Ferulic acid (FA), a component of hemicellulose in plant cell walls, is a phenolic acid with several potential applications based on its antioxidant properties. Recent studies have shown that feruloyl esterase (FAE) is a key bacterial enzyme involved in FA production from agricultural biomass. In this study, we screened a library of 43 esterases from Streptomyces species and identified two enzymes, R18 and R43, that have FAE activity toward ethyl ferulate. In addition, we characterized their enzyme properties in detail. R18 and R43 showed esterase activity toward other hydroxycinnamic acid esters as well, such as methyl p-coumarate, methyl caffeate, and methyl sinapinate. The amino acid sequences of R18 and R43 were neither similar to each other, nor to other FAEs. We found that R18 and R43 individually showed the ability to produce FA from corn bran; however, combination with other Streptomyces enzymes, namely xylanase and alpha-l-arabinofuranosidase, increased FA production from biomass such as corn bran, defatted rice bran, and wheat bran. These results suggest that R18 and R43 are effective FAEs for the enzymatic production of FA from biomass.

PubMedSearch : Uraji_2014_PLoS.One_9_e104584
PubMedID: 25093500
Gene_locus related to this paper: strcj-estA , strcj-estB

Related information

Substrate Sinapinic-acid
Gene_locus Sinapinic-acid    strcj-estA    strcj-estB

Citations formats

Uraji M, Arima J, Inoue Y, Harazono K, Hatanaka T (2014)
Application of two newly identified and characterized feruloyl esterases from Streptomyces sp. in the enzymatic production of ferulic acid from agricultural biomass
PLoS ONE 9 :e104584

Uraji M, Arima J, Inoue Y, Harazono K, Hatanaka T (2014)
PLoS ONE 9 :e104584