| Title : [Effective binding of alpha-bungarotoxin with the solubilized alpha-subunit of Torpedo californica acetylcholine receptor] - Utkin_1996_Bioorg.Khim_22_387 |
| Author(s) : Utkin Iu N , Mund M , Hucho F , Tsetlin VI |
| Ref : Bioorganicheskaia Khimiia , 22 :387 , 1996 |
|
Abstract :
alpha-Subunit of the Torpedo californica nicotinic acetylcholine receptor was isolated by preparative SDS-PAGE followed by reversed-phase HPLC on a C4 column in an acetonitrile-isopropanol gradient in water. After removal of the organic solvents and solubilization in beta-octylglucoside, the purified alpha-subunit binds alpha-bungarotoxin with high affinity (Kd 28 nM). |
| PubMedSearch : Utkin_1996_Bioorg.Khim_22_387 |
| PubMedID: 8929226 |
Utkin Iu N, Mund M, Hucho F, Tsetlin VI (1996)
[Effective binding of alpha-bungarotoxin with the solubilized alpha-subunit of Torpedo californica acetylcholine receptor]
Bioorganicheskaia Khimiia
22 :387
Utkin Iu N, Mund M, Hucho F, Tsetlin VI (1996)
Bioorganicheskaia Khimiia
22 :387