Verschoor_2024_Commun.Biol_7_725

Reference

Title : Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces - Verschoor_2024_Commun.Biol_7_725
Author(s) : Verschoor JA , Croese MRJ , Lakemeier SE , Mugge A , Burgers CMC , Innocenti P , Willemse J , Crooijmans ME , van Wezel GP , Ram AFJ , de Winde JH
Ref : Commun Biol , 7 :725 , 2024
Abstract :

The rising use of plastic results in an appalling amount of waste which is scattered into the environment. One of these plastics is PET which is mainly used for bottles. We have identified and characterized an esterase from Streptomyces, annotated as LipA, which can efficiently degrade the PET-derived oligomer BHET. The Streptomyces coelicolor ScLipA enzyme exhibits varying sequence similarity to several BHETase/PETase enzymes, including IsPETase, TfCut2, LCC, PET40 and PET46. Of 96 Streptomyces strains, 18% were able to degrade BHET via one of three variants of LipA, named ScLipA, S2LipA and S92LipA. SclipA was deleted from S. coelicolor resulting in reduced BHET degradation. Overexpression of all LipA variants significantly enhanced BHET degradation. All variants were expressed in E. coli for purification and biochemical analysis. The optimum conditions were determined as pH 7 and 25 degreesC for all variants. The activity on BHET and amorphous PET film was investigated. S2LipA efficiently degraded BHET and caused roughening and indents on the surface of PET films, comparable to the activity of previously described TfCut2 under the same conditions. The abundance of the S2LipA variant in Streptomyces suggests an environmental advantage towards the degradation of more polar substrates including these polluting plastics.

PubMedSearch : Verschoor_2024_Commun.Biol_7_725
PubMedID: 38867087
Gene_locus related to this paper: strco-LIPA

Related information

Substrate BHET
Gene_locus strco-LIPA

Citations formats

Verschoor JA, Croese MRJ, Lakemeier SE, Mugge A, Burgers CMC, Innocenti P, Willemse J, Crooijmans ME, van Wezel GP, Ram AFJ, de Winde JH (2024)
Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces
Commun Biol 7 :725

Verschoor JA, Croese MRJ, Lakemeier SE, Mugge A, Burgers CMC, Innocenti P, Willemse J, Crooijmans ME, van Wezel GP, Ram AFJ, de Winde JH (2024)
Commun Biol 7 :725