Title : Characterization of muscarinic receptors in human pancreatic membranes - Waelbroeck_1988_Pancreas_3_627 |
Author(s) : Waelbroeck M , Camus J , Tastenoy M , de Neef P , Scemama JL , Fourmy D , Vaysse N , Pradayrol L , Robberecht P , Christophe J |
Ref : Pancreas , 3 :627 , 1988 |
Abstract :
Crude membranes (27,000 x g pellets) from three normal human pancreata were prepared. Muscarinic receptors were investigated by the ability of three antagonists (atropine, pirenzepine, and AF-DX 116) and three agonists (carbamylcholine, oxotremorine, and pilocarpine) to inhibit [3H]NMS binding. These receptors showed for pirenzepine and AF-DX 116 a M2 beta specificity, typical of secretory glands and smooth muscle, that was comparable to that of rat pancreatic membranes, i.e., a low affinity for the two antagonists (Ki of 0.4 and 0.2 microM, respectively). In addition, these receptors were predominantly in a low affinity state for the agonist carbamylcholine (Ki of 100 microM). |
PubMedSearch : Waelbroeck_1988_Pancreas_3_627 |
PubMedID: 3222245 |
Waelbroeck M, Camus J, Tastenoy M, de Neef P, Scemama JL, Fourmy D, Vaysse N, Pradayrol L, Robberecht P, Christophe J (1988)
Characterization of muscarinic receptors in human pancreatic membranes
Pancreas
3 :627
Waelbroeck M, Camus J, Tastenoy M, de Neef P, Scemama JL, Fourmy D, Vaysse N, Pradayrol L, Robberecht P, Christophe J (1988)
Pancreas
3 :627