Walsh_2008_J.Biol.Chem_283_6022

Reference

Title : Up-regulation of nicotinic receptors by nicotine varies with receptor subtype - Walsh_2008_J.Biol.Chem_283_6022
Author(s) : Walsh H , Govind AP , Mastro R , Hoda JC , Bertrand D , Vallejo Y , Green WN
Ref : Journal of Biological Chemistry , 283 :6022 , 2008
Abstract :

Recent evidence suggests that in addition to alpha4beta2 and alpha3-containing nicotinic receptors, alpha6-containing receptors are present in midbrain dopaminergic neurons and involved in the nicotine reward pathway. Using heterologous expression, we found that alpha6beta2, like alpha3beta2 and alpha4beta2 receptors, formed high affinity epibatidine binding complexes that are pentameric, trafficked to the cell surface, and produced acetylcholine-evoked currents. Chronic nicotine exposure up-regulated alpha6beta2 receptors with differences in up-regulation time course and concentration dependence compared with alpha4beta2 receptors, the predominant high affinity nicotine binding site in brain. The alpha6beta2 receptor up-regulation required higher nicotine concentrations than for alpha4beta2 but lower than for alpha3beta2 receptors. The alpha6beta2 up-regulation occurred 10-fold faster than for alpha4beta2 and slightly faster than for alpha3beta2. Our data suggest that nicotinic receptor up-regulation is subtype-specific such that alpha6-containing receptors up-regulate in response to transient, high nicotine exposures, whereas sustained, low nicotine exposures up-regulate alpha4beta2 receptors.

PubMedSearch : Walsh_2008_J.Biol.Chem_283_6022
PubMedID: 18174175

Related information

Citations formats

Walsh H, Govind AP, Mastro R, Hoda JC, Bertrand D, Vallejo Y, Green WN (2008)
Up-regulation of nicotinic receptors by nicotine varies with receptor subtype
Journal of Biological Chemistry 283 :6022

Walsh H, Govind AP, Mastro R, Hoda JC, Bertrand D, Vallejo Y, Green WN (2008)
Journal of Biological Chemistry 283 :6022