| Title : A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme - Watanabe_2015_Process.Biochem_50_1718 |
| Author(s) : Watanabe T , Suzuki K , Shinozaki Y , Yarimizu T , Yoshida S , Sameshima-Yamashita Y , Koitabashi M , Kitamoto HK |
| Ref : Process Biochemistry , 50 :1718 , 2013 |
|
Abstract :
Poly(L-lactic acid) (PLA)-degrading Pseudomonas sp. strain DS04-T was isolated from an activated sludge sample. A novel PLA depolymerase with a molecular weight of 34 kDa was purified to homogeneity from the culture supernatant of strain DS04-T. The optimum pH and temperature of the PLA depolymerase are 8.5 and 50C, respectively. The PLA depolymerase can also degrade triolein, poly(-hydroxybutyrate), and poly(e-caprolactone). Na+ and K+ enhance the enzyme activity, whereas Zn2+, Mg2+, Cu2+, and Fe2+ inhibit it. The inhibition effect of different chemicals on the PLA depolymerase activity were examined, in which ethylenediaminetetraacetic acid was found to have a significant inhibitory effect. The main degradation product of the depolymerase is identified as lactic acid monomer by mass spectrometric analysis |
| PubMedSearch : Watanabe_2015_Process.Biochem_50_1718 |
| PubMedID: |
| Substrate | Polylactic-acid |
Watanabe T, Suzuki K, Shinozaki Y, Yarimizu T, Yoshida S, Sameshima-Yamashita Y, Koitabashi M, Kitamoto HK (2013)
A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme
Process Biochemistry
50 :1718
Watanabe T, Suzuki K, Shinozaki Y, Yarimizu T, Yoshida S, Sameshima-Yamashita Y, Koitabashi M, Kitamoto HK (2013)
Process Biochemistry
50 :1718