Wiemann_2013_PLoS.Pathog_9_e1003475

Reference

Title : Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites - Wiemann_2013_PLoS.Pathog_9_e1003475
Author(s) : Wiemann P , Sieber CM , von Bargen KW , Studt L , Niehaus EM , Espino JJ , Huss K , Michielse CB , Albermann S , Wagner D , Bergner SV , Connolly LR , Fischer A , Reuter G , Kleigrewe K , Bald T , Wingfield BD , Ophir R , Freeman S , Hippler M , Smith KM , Brown DW , Proctor RH , Munsterkotter M , Freitag M , Humpf HU , Guldener U , Tudzynski B
Ref : PLoS Pathog , 9 :e1003475 , 2013
Abstract :

The fungus Fusarium fujikuroi causes "bakanae" disease of rice due to its ability to produce gibberellins (GAs), but it is also known for producing harmful mycotoxins. However, the genetic capacity for the whole arsenal of natural compounds and their role in the fungus' interaction with rice remained unknown. Here, we present a high-quality genome sequence of F. fujikuroi that was assembled into 12 scaffolds corresponding to the 12 chromosomes described for the fungus. We used the genome sequence along with ChIP-seq, transcriptome, proteome, and HPLC-FTMS-based metabolome analyses to identify the potential secondary metabolite biosynthetic gene clusters and to examine their regulation in response to nitrogen availability and plant signals. The results indicate that expression of most but not all gene clusters correlate with proteome and ChIP-seq data. Comparison of the F. fujikuroi genome to those of six other fusaria revealed that only a small number of gene clusters are conserved among these species, thus providing new insights into the divergence of secondary metabolism in the genus Fusarium. Noteworthy, GA biosynthetic genes are present in some related species, but GA biosynthesis is limited to F. fujikuroi, suggesting that this provides a selective advantage during infection of the preferred host plant rice. Among the genome sequences analyzed, one cluster that includes a polyketide synthase gene (PKS19) and another that includes a non-ribosomal peptide synthetase gene (NRPS31) are unique to F. fujikuroi. The metabolites derived from these clusters were identified by HPLC-FTMS-based analyses of engineered F. fujikuroi strains overexpressing cluster genes. In planta expression studies suggest a specific role for the PKS19-derived product during rice infection. Thus, our results indicate that combined comparative genomics and genome-wide experimental analyses identified novel genes and secondary metabolites that contribute to the evolutionary success of F. fujikuroi as a rice pathogen.

PubMedSearch : Wiemann_2013_PLoS.Pathog_9_e1003475
PubMedID: 23825955
Gene_locus related to this paper: fusox-a0a1d3s5h0 , fusof-f9f6t8 , fusof-f9f6v2 , fusof-f9f132 , fusof-f9fd90 , fusof-f9fmx2 , fusof-f9fnt4 , fusof-f9g2a2 , fusof-f9g797 , fusof-f9ga50 , fusof-f9gck4 , fusof-f9gd15 , gibf5-s0ea65 , gibf5-s0enx6 , gibf5-s0epd5 , gibf5-s0dwm1 , gibf5-s0egd1 , gibf5-s0duw7 , gibf5-s0ekf7 , gibf5-s0e2n9 , gibf5-s0en31 , gibf5-s0e7t9 , gibf5-s0eim9 , gibf5-s0drv8 , gibf5-s0e9e0 , gibf5-s0e3u9 , gibf5-s0dzu5 , gibf5-s0dqr2 , gibf5-s0ecj3 , gibf5-s0ekc4 , gibf5-s0ejq7 , gibf5-s0eli0 , gibf5-s0dz40 , gibf5-s0ehu3 , gibf5-fuj3 , gibf5-fub5 , gibf5-fus5 , gibf5-bik1

Related information

Gene_locus fusox-a0a1d3s5h0    fusof-f9f6t8    fusof-f9f6v2    fusof-f9f132    fusof-f9fd90    fusof-f9fmx2    fusof-f9fnt4    fusof-f9g2a2    fusof-f9g797    fusof-f9ga50    fusof-f9gck4    fusof-f9gd15    gibf5-s0ea65    gibf5-s0enx6    gibf5-s0epd5    gibf5-s0dwm1    gibf5-s0egd1    gibf5-s0duw7    gibf5-s0ekf7    gibf5-s0e2n9    gibf5-s0en31    gibf5-s0e7t9    gibf5-s0eim9    gibf5-s0drv8    gibf5-s0e9e0    gibf5-s0e3u9    gibf5-s0dzu5    gibf5-s0dqr2    gibf5-s0ecj3    gibf5-s0ekc4    gibf5-s0ejq7    gibf5-s0eli0    gibf5-s0dz40    gibf5-s0ehu3    gibf5-fuj3    gibf5-fub5    gibf5-fus5    gibf5-bik1
Gene_locus_frgt fusox-a0a1d3s5h0    fusof-f9f6t8    fusof-f9f6v2    fusof-f9f132    fusof-f9fd90    fusof-f9fmx2    fusof-f9fnt4    fusof-f9g2a2    fusof-f9g797    fusof-f9ga50    fusof-f9gck4    fusof-f9gd15    gibf5-s0ea65    gibf5-s0enx6    gibf5-s0epd5    gibf5-s0dwm1    gibf5-s0egd1    gibf5-s0duw7    gibf5-s0ekf7    gibf5-s0e2n9    gibf5-s0en31    gibf5-s0e7t9    gibf5-s0eim9    gibf5-s0drv8    gibf5-s0e9e0    gibf5-s0e3u9    gibf5-s0dzu5    gibf5-s0dqr2    gibf5-s0ecj3    gibf5-s0ekc4    gibf5-s0ejq7    gibf5-s0eli0    gibf5-s0dz40    gibf5-s0ehu3    gibf5-fuj3    gibf5-fub5    gibf5-fus5    gibf5-bik1    gibf5-s0e1q0    gibf5-s0e264

Citations formats

Wiemann P, Sieber CM, von Bargen KW, Studt L, Niehaus EM, Espino JJ, Huss K, Michielse CB, Albermann S, Wagner D, Bergner SV, Connolly LR, Fischer A, Reuter G, Kleigrewe K, Bald T, Wingfield BD, Ophir R, Freeman S, Hippler M, Smith KM, Brown DW, Proctor RH, Munsterkotter M, Freitag M, Humpf HU, Guldener U, Tudzynski B (2013)
Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites
PLoS Pathog 9 :e1003475

Wiemann P, Sieber CM, von Bargen KW, Studt L, Niehaus EM, Espino JJ, Huss K, Michielse CB, Albermann S, Wagner D, Bergner SV, Connolly LR, Fischer A, Reuter G, Kleigrewe K, Bald T, Wingfield BD, Ophir R, Freeman S, Hippler M, Smith KM, Brown DW, Proctor RH, Munsterkotter M, Freitag M, Humpf HU, Guldener U, Tudzynski B (2013)
PLoS Pathog 9 :e1003475