| Title : Biochemistry detection of acetylcholinesterase activity in Trypanosoma evansi and possible functional correlations - Wolkmer_2012_Exp.Parasitol_132_546 |
| Author(s) : Wolkmer P , da Silva CB , Paim FC , Da Silva AS , Tavares KC , Lazzarotto CR , Palma HE , Thome GR , Miletti LC , Schetinger MR , Lopes ST , Mazzanti CM |
| Ref : Experimental Parasitology , 132 :546 , 2012 |
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Abstract :
Several chemical and immunohistochemical techniques can be used for the detection of acetylcholinesterase (AChE) activity. In this experiment we aimed to detect AChE activity in Trypanosoma evansi. For this, the parasites were isolated from the blood of experimentally infected rats using a DEA-cellulose column. Enzymatic activity was determined in trypomastigote forms at 0, 0.2, 0.4, 0.8 and 1.2mg/mL of protein concentrations by a standard biochemical protocol. At all concentrations tested, the study showed that T. evansi expresses the enzyme AChE and its activity was proportional to the concentration of protein, ranging between 0.64 and 2.70mumol of AcSCh/h. Therefore, we concluded that it is possible to biochemically detect AChE in T. evansi, an enzyme that may be associated with vital functions of the parasite and also can be related to chemotherapy treatments, as further discussed in this article. |
| PubMedSearch : Wolkmer_2012_Exp.Parasitol_132_546 |
| PubMedID: 22981718 |
Wolkmer P, da Silva CB, Paim FC, Da Silva AS, Tavares KC, Lazzarotto CR, Palma HE, Thome GR, Miletti LC, Schetinger MR, Lopes ST, Mazzanti CM (2012)
Biochemistry detection of acetylcholinesterase activity in Trypanosoma evansi and possible functional correlations
Experimental Parasitology
132 :546
Wolkmer P, da Silva CB, Paim FC, Da Silva AS, Tavares KC, Lazzarotto CR, Palma HE, Thome GR, Miletti LC, Schetinger MR, Lopes ST, Mazzanti CM (2012)
Experimental Parasitology
132 :546